Bio Spec Overview
Ignite Creation Date:
2025-12-24 @ 1:47 PM
Last Modification Date:
2025-12-24 @ 1:47 PM
Retention:
SAMPLES_WITH_DNA
Description:
Tissue Dissociation
The tissue from the plates will be enzymatically dissociated into single-cell suspensions using a combination of collagenase, DNase I, and dispase.
Cells will be filtered through a 70 µm filter to remove debris and aggregates. Viability Check and Enrichment
Cell viability will be assessed using Trypan blue exclusion. Dead cells and debris will be removed using a magnetic kit for dead cell removal, if necessary.
scRNAseq Workflow Library Preparation
Single-cell capture and library preparation will be performed using a droplet-based microfluidic system (e.g., 10x Genomics Chromium).
Approximately 5,000-10,000 cells per sample will be targeted for analysis.
The tissue from the plates will be enzymatically dissociated into single-cell suspensions using a combination of collagenase, DNase I, and dispase.
Cells will be filtered through a 70 µm filter to remove debris and aggregates. Viability Check and Enrichment
Cell viability will be assessed using Trypan blue exclusion. Dead cells and debris will be removed using a magnetic kit for dead cell removal, if necessary.
scRNAseq Workflow Library Preparation
Single-cell capture and library preparation will be performed using a droplet-based microfluidic system (e.g., 10x Genomics Chromium).
Approximately 5,000-10,000 cells per sample will be targeted for analysis.
Section:
Tissue Dissociation
The tissue from the plates will be enzymatically dissociated into single-cell suspensions using a combination of collagenase, DNase I, and dispase.
Cells will be filtered through a 70 µm filter to remove debris and aggregates. Viability Check and Enrichment
Cell viability will be assessed using Trypan blue exclusion. Dead cells and debris will be removed using a magnetic kit for dead cell removal, if necessary.
scRNAseq Workflow Library Preparation
Single-cell capture and library preparation will be performed using a droplet-based microfluidic system (e.g., 10x Genomics Chromium).
Approximately 5,000-10,000 cells per sample will be targeted for analysis.