Bio Spec Overview
Ignite Creation Date:
2025-12-24 @ 2:20 PM
Last Modification Date:
2025-12-24 @ 2:20 PM
Retention:
SAMPLES_WITH_DNA
Description:
In this study, the qualified specimens will be obtained by biopsy, scraping, and swab for HPV DNA detection. Cancer tissue will be taken from patients with anal cancers. For surgical patients, cancer tissue can be obtained by biopsy or retrospective paraffin embedded tissue, for non-surgical patients, tissue can be obtained by biopsy. For tissue samples, cut about 5-25mg of the sample, put it into a 1.5ml centrifuge tube, add 180 Buffer AL and 20ul proteinase K solution, shake and mix well, centrifuge at low speed, and then store for testing. For retrospective paraffin embedded specimens, use a scalpel to remove excess paraffin and cut a 10um thick slice. If the surface of the specimen is exposed to air, discard the first 2-3 slices. Disassemble the microtome between blocks for decontamination.
Section:
In this study, the qualified specimens will be obtained by biopsy, scraping, and swab for HPV DNA detection. Cancer tissue will be taken from patients with anal cancers. For surgical patients, cancer tissue can be obtained by biopsy or retrospective paraffin embedded tissue, for non-surgical patients, tissue can be obtained by biopsy. For tissue samples, cut about 5-25mg of the sample, put it into a 1.5ml centrifuge tube, add 180 Buffer AL and 20ul proteinase K solution, shake and mix well, centrifuge at low speed, and then store for testing. For retrospective paraffin embedded specimens, use a scalpel to remove excess paraffin and cut a 10um thick slice. If the surface of the specimen is exposed to air, discard the first 2-3 slices. Disassemble the microtome between blocks for decontamination.