Ignite Creation Date:
2024-05-05 @ 11:34 AM
Last Modification Date:
2024-10-26 @ 9:10 AM
Study NCT ID:
NCT00087919
Status:
COMPLETED
Last Update Posted:
2022-02-23
First Post:
2004-07-15
Brief Title:
Defining an Obesity QTL on Chromosome 3q
Sponsor:
Case Western Reserve University
Organization:
Case Western Reserve University
Study Overview
Official Title:
None
Status:
COMPLETED
Status Verified Date:
2008-07
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
To investigate the genetic basis of obesity by fine mapping an obesity quantitative trail linkage QTL linked to chromosome 3q
Detailed Description:
BACKGROUND
In the last several decades obesity has emerged as a major public health threat While prevention through lifestyle change is the only long-term solution better understanding of the physiologic mechanisms would greatly assist development of drugs and targeted prevention Obesity is a highly heritable condition and while genes must account for a substantial proportion of individual susceptibility they have eluded detection Powerful new genetic and genomic tools now permit comprehensive evaluation of candidate genes including all genes under linkage peaks These tools include new genomic resources the human genome sequence databases of common SNPs and the haplotype map rapid and inexpensive discovery and genotyping and new analytic methods haplotype-based association and admixture mapping
DESIGN NARRATIVE
In a large African American family set Dr Zhu and colleagues have obtained strong linkage evidence for obesity on chromosome 3q combined LOD score 37 A prime candidate adiponectin lies near this peak They propose to follow up that finding by combining the epidemiologic data with high-throughput genotyping and move from linkage to association analysis The results for this QTL will be evaluated within the available environmental factors to assess potential gene-environment and gene-gene interactions The available phenotypes include body composition resting metabolic rate physical activity plasma insulin glucose and leptin In a family-based design they will examine the linkage peak centered on position 188 cM on chromosome 3q 20 Cm 1-LOD support interval with the following step-wise strategy a Genotype 200 single nucleotide polymorphisms SNPs in this region on 300 families 1000 individuals b Conduct linkage linkage disequilibrium and admixture mapping to potentially further narrow the region and c Conduct resequencing and haplotype-based association studies for all candidate genes under the peak Statistical analysis incorporating intermediate phenotypes and environmental covariates will be used to characterize potential gene x gene or gene x environment interactions Replication will be tested in additional populations of African and European origin
In the last several decades obesity has emerged as a major public health threat While prevention through lifestyle change is the only long-term solution better understanding of the physiologic mechanisms would greatly assist development of drugs and targeted prevention Obesity is a highly heritable condition and while genes must account for a substantial proportion of individual susceptibility they have eluded detection Powerful new genetic and genomic tools now permit comprehensive evaluation of candidate genes including all genes under linkage peaks These tools include new genomic resources the human genome sequence databases of common SNPs and the haplotype map rapid and inexpensive discovery and genotyping and new analytic methods haplotype-based association and admixture mapping
DESIGN NARRATIVE
In a large African American family set Dr Zhu and colleagues have obtained strong linkage evidence for obesity on chromosome 3q combined LOD score 37 A prime candidate adiponectin lies near this peak They propose to follow up that finding by combining the epidemiologic data with high-throughput genotyping and move from linkage to association analysis The results for this QTL will be evaluated within the available environmental factors to assess potential gene-environment and gene-gene interactions The available phenotypes include body composition resting metabolic rate physical activity plasma insulin glucose and leptin In a family-based design they will examine the linkage peak centered on position 188 cM on chromosome 3q 20 Cm 1-LOD support interval with the following step-wise strategy a Genotype 200 single nucleotide polymorphisms SNPs in this region on 300 families 1000 individuals b Conduct linkage linkage disequilibrium and admixture mapping to potentially further narrow the region and c Conduct resequencing and haplotype-based association studies for all candidate genes under the peak Statistical analysis incorporating intermediate phenotypes and environmental covariates will be used to characterize potential gene x gene or gene x environment interactions Replication will be tested in additional populations of African and European origin
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| R01HL074166 | NIH | None | httpsreporternihgovquickSearchR01HL074166 |