Ignite Creation Date:
2024-05-05 @ 11:35 AM
Last Modification Date:
2024-10-26 @ 9:10 AM
Study NCT ID:
NCT00082225
Status:
TERMINATED
Last Update Posted:
2017-02-07
First Post:
2004-05-03
Brief Title:
LMP2a-Specific Cytotoxic T-Lymphocytes Lymphoma ACDAL
Sponsor:
Baylor College of Medicine
Organization:
Baylor College of Medicine
Study Overview
Official Title:
Administration of LMP2a-Specific Cytotoxic T-Lymphocytes Following CD45 Antibody to Patients With Relapsed EBV-Positive Hodgkins or Non-Hodgkins Lymphoma
Status:
TERMINATED
Status Verified Date:
2017-02
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
ACDAL
Brief Summary:
Patients have a type of cancer called Hodgkins lymphoma or non-Hodgkins lymphoma which has come back or not gone away or is at high risk for coming back after treatment including the best treatment investigators know for this disease Investigators are asking the patient to volunteer to be in a research study using a new experimental therapy consisting of special immune system cells called LMP2 specific cytotoxic T lymphocytes in combination with a special protein called a monoclonal antibody
Some patients with Hodgkin or non-Hodgkin Lymphoma show evidence of infection with the virus that causes infectious mononucleosis Epstein Barr virus EBV before or at the time of their diagnosis of the lymphoma EBV is found in the cancer cells of up to half the patients with lymphoma suggesting that it may play a role in causing lymphoma The cancer cells infected by EBV are able to hide from the bodys immune system and escape destruction Investigators want to see if special white blood cells called T cells that have been trained to kill EBV infected cells can survive in the blood and affect the tumor
The investigators have used this sort of therapy to treat a different type of cancer that occurs after bone marrow and solid organ transplant called post transplant lymphoma In this type of cancer the tumor cells have 9 proteins made by EBV on their surface They grew T cells in the laboratory that recognized all 9 proteins and were able to prevent and treat post transplant lymphoma However in Hodgkin disease and non-Hodgkin Lymphoma the tumor cells only express 2 EBV proteins In a previous study investigators made T cells that recognized all 9 proteins and gave them to patients with Hodgkin disease Some patients had a partial response to this therapy but no patients had a complete response Investigators think one reason may be that many of the T cells reacted with proteins that were not on the tumor cells They are now trying to find out if they can improve this treatment by growing T cells that only recognize one of the proteins expressed on Lymphoma cells called LMP-2 These special T cells are called LMP-2 specific cytotoxic T-lymphocytes CTLs
In this study investigators also are trying to see if they can improve these results by treating patients first with a special protein called an antibody and then giving the EBV specific T cells The reason for doing this is that EBV specific T cells have worked very well in bone marrow transplant patients to prevent and treat EBV cancers These patients have very few of their own immune cells when they are given the trained T cells and therefore there is a lot of space for the trained cells to grow Investigators hope that they can improve the effect of the trained T cells in Hodgkin disease and non-Hodgkin Lymphoma patients by first temporarily removing the patients own T cells before giving the trained cells
Some patients with Hodgkin or non-Hodgkin Lymphoma show evidence of infection with the virus that causes infectious mononucleosis Epstein Barr virus EBV before or at the time of their diagnosis of the lymphoma EBV is found in the cancer cells of up to half the patients with lymphoma suggesting that it may play a role in causing lymphoma The cancer cells infected by EBV are able to hide from the bodys immune system and escape destruction Investigators want to see if special white blood cells called T cells that have been trained to kill EBV infected cells can survive in the blood and affect the tumor
The investigators have used this sort of therapy to treat a different type of cancer that occurs after bone marrow and solid organ transplant called post transplant lymphoma In this type of cancer the tumor cells have 9 proteins made by EBV on their surface They grew T cells in the laboratory that recognized all 9 proteins and were able to prevent and treat post transplant lymphoma However in Hodgkin disease and non-Hodgkin Lymphoma the tumor cells only express 2 EBV proteins In a previous study investigators made T cells that recognized all 9 proteins and gave them to patients with Hodgkin disease Some patients had a partial response to this therapy but no patients had a complete response Investigators think one reason may be that many of the T cells reacted with proteins that were not on the tumor cells They are now trying to find out if they can improve this treatment by growing T cells that only recognize one of the proteins expressed on Lymphoma cells called LMP-2 These special T cells are called LMP-2 specific cytotoxic T-lymphocytes CTLs
In this study investigators also are trying to see if they can improve these results by treating patients first with a special protein called an antibody and then giving the EBV specific T cells The reason for doing this is that EBV specific T cells have worked very well in bone marrow transplant patients to prevent and treat EBV cancers These patients have very few of their own immune cells when they are given the trained T cells and therefore there is a lot of space for the trained cells to grow Investigators hope that they can improve the effect of the trained T cells in Hodgkin disease and non-Hodgkin Lymphoma patients by first temporarily removing the patients own T cells before giving the trained cells
Detailed Description:
Investigators first tested a biopsy of the tumor that has already been done to see if the tumor cells are EBV positive They then got permission to take up to 60ml 12 teaspoonfuls of blood from the patient or their donor on one or two occasions and used this blood to grow T cells in the laboratory They first grew a special type of cell called dendritic cells stimulate the T cells and put a specially produced human virus adenovirus that carries the LMP-2a gene into the dendritic cells These dendritic cells were then treated with radiation so they could not grow They were then used to stimulate T cells This stimulation trained the T cells to kill cells with LMP-2a on their surface Investigators then grew these LMP-2a specific CTLs by more stimulation with EBV infected cells which were made from the patients blood or their donors blood by infecting them with EBV in the laboratory The investigators also put the adenovirus that carries the LMP2 gene into these EBV infected cells to increase the amount of LMP2 that these cells have Again these EBV infected cells were treated with radiation so they could not grow Once sufficient numbers of T cells were made investigators tested them to make sure they kill cells with LMP2a on their surface These cells are now ready to give to the patient if they agree to being on this study
Investigators also took up to 500 ml 2 12 cups of extra blood from the patient or their donor which were frozen In case the patients own cells do not recover as expected after the antibody and cell infusions these cells can be thawed and given back to the patient
If the patient agrees to this treatment they will get treated with the CD45 antibodies for 4 days in a row and then 2-3 days later get a dose of LMP2 specific CTLs
The CD45 antibodies will be given to the patient through a vein for 6-8 hours and monitored for at least 6 hours after the infusion After the infusion The patient will be checked for the levels of CD45 in the blood at 24 hours optional andor at 48-72 hours after the last infusion to check the level is low enough to give the patient the CTLs
The CTLs will be thawed and injected through a central line if the patient has one or through a vein in their arm over 10 minutes after pretreatment with Tylenol and Benadryl We will then monitor them in clinic for 4 hours after the injection
All of the treatments will be given by the Center for Cell and Gene Therapy at Texas Childrens Hospital or the Methodist Hospital We will follow the patient in the clinic after the CTL injection If there is a reduction in the size of the lymphoma on CT or MRI scans as assessed by a radiologist they can receive up to six additional doses of the T cells if they wish
Investigators also took up to 500 ml 2 12 cups of extra blood from the patient or their donor which were frozen In case the patients own cells do not recover as expected after the antibody and cell infusions these cells can be thawed and given back to the patient
If the patient agrees to this treatment they will get treated with the CD45 antibodies for 4 days in a row and then 2-3 days later get a dose of LMP2 specific CTLs
The CD45 antibodies will be given to the patient through a vein for 6-8 hours and monitored for at least 6 hours after the infusion After the infusion The patient will be checked for the levels of CD45 in the blood at 24 hours optional andor at 48-72 hours after the last infusion to check the level is low enough to give the patient the CTLs
The CTLs will be thawed and injected through a central line if the patient has one or through a vein in their arm over 10 minutes after pretreatment with Tylenol and Benadryl We will then monitor them in clinic for 4 hours after the injection
All of the treatments will be given by the Center for Cell and Gene Therapy at Texas Childrens Hospital or the Methodist Hospital We will follow the patient in the clinic after the CTL injection If there is a reduction in the size of the lymphoma on CT or MRI scans as assessed by a radiologist they can receive up to six additional doses of the T cells if they wish
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| ACDAL | OTHER | Baylor College of Medicine | None |