Ignite Creation Date:
2024-05-05 @ 11:50 AM
Last Modification Date:
2024-10-26 @ 9:15 AM
Study NCT ID:
NCT00155051
Status:
UNKNOWN
Last Update Posted:
2005-09-12
First Post:
2005-09-09
Brief Title:
Progestin Treatment for Endometrial Stromal Cells in Adenomyosis
Sponsor:
National Taiwan University Hospital
Organization:
National Taiwan University Hospital
Study Overview
Official Title:
None
Status:
UNKNOWN
Status Verified Date:
2004-06
Last Known Status:
RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Long term treatment of progestin has been demonstrated to have an inhibitory effect on endometrial angiogenesis and the proliferation of endometrial stromal cells As a result progestin is now widely employed in the treatment of endometrial cancer endometrial hyperplasia and dysfunction uterine bleeding In the treatment of adenomyosis however the beneficial effect of progestin was limited It might imply that the behavior of endometrial cells in women with adenomyosis is different from that in women without adenomyosis
Our previous study revealed that the expression of killer inhibitory receptors KIRs on NK cells was decreased in eutopic endometrium in women with adenomyosis It may be a compensatory effect in which the NK cytotoxicity is activated in order to wipe out the abnormal endometrial cells that might go out of the eutopic site of endometrium It implies that the formation of adenomyosis might be due to abnormal endometrial tissues but not the aberrant local immunological dysfunction in myometrium This finding is compatible with previous reports in which eutopic endometrium obtained from women with endometriosis or adenomyosis was found to behave differently from endometrium in unaffected women
In this study we try to collect endometrial tissues from women with and without adenomyosis and then purify the endometrial stromal cells from endometrium The endometrial stromal cells are cultured for 8 days with the supplement of medroxyprogesterone MPA or danazol Quantification of IL-6 and IL-8 mRNA in endometrial cells and the concentrations of IL-6 and IL-8 in cultured media will be done with real time RT-PCR and ELISA respectively The expression of different cytokines of endometrial cells in response to progestin might be further elucidated after our experiment
Our previous study revealed that the expression of killer inhibitory receptors KIRs on NK cells was decreased in eutopic endometrium in women with adenomyosis It may be a compensatory effect in which the NK cytotoxicity is activated in order to wipe out the abnormal endometrial cells that might go out of the eutopic site of endometrium It implies that the formation of adenomyosis might be due to abnormal endometrial tissues but not the aberrant local immunological dysfunction in myometrium This finding is compatible with previous reports in which eutopic endometrium obtained from women with endometriosis or adenomyosis was found to behave differently from endometrium in unaffected women
In this study we try to collect endometrial tissues from women with and without adenomyosis and then purify the endometrial stromal cells from endometrium The endometrial stromal cells are cultured for 8 days with the supplement of medroxyprogesterone MPA or danazol Quantification of IL-6 and IL-8 mRNA in endometrial cells and the concentrations of IL-6 and IL-8 in cultured media will be done with real time RT-PCR and ELISA respectively The expression of different cytokines of endometrial cells in response to progestin might be further elucidated after our experiment
Detailed Description:
Eutopic endometrium was obtained and separated into single endometrial stromal cell ESC in women with adenomyosis study group and without adenomyosis control group
After becoming pre-confluent covering 80 of the culture well ESC was cultured for 8 days solely or with the addition of medroxyprogesterone MPA or danazol
ELISA was done to measure IL-6 IL-8 and TNF-alpha concentrations of the culture media
Real-time quantitative RT-PCR was done to measure IL-6 IL-8 and TNF-alpha RNA levels in ESC
After becoming pre-confluent covering 80 of the culture well ESC was cultured for 8 days solely or with the addition of medroxyprogesterone MPA or danazol
ELISA was done to measure IL-6 IL-8 and TNF-alpha concentrations of the culture media
Real-time quantitative RT-PCR was done to measure IL-6 IL-8 and TNF-alpha RNA levels in ESC
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| NTUH94S72 | None | None | None |