Ignite Creation Date:
2024-05-05 @ 11:52 AM
Last Modification Date:
2024-10-26 @ 9:15 AM
Study NCT ID:
NCT00154453
Status:
COMPLETED
Last Update Posted:
2005-09-12
First Post:
2005-09-09
Brief Title:
Age-Related Changes in Myosin Heavy Chain Composition in Human Orbicularis Oculi Muscle
Sponsor:
National Taiwan University Hospital
Organization:
National Taiwan University Hospital
Study Overview
Official Title:
None
Status:
COMPLETED
Status Verified Date:
2003-10
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Orbicularis oculi muscle is a key structure in the peri-orbital anatomy and is responsible for the major changes of aging in this area Peri-orbital rejuvenation has been an issue of great interest in plastic surgery We believe that understanding the age-related histomorphometric changes in orbicularis oculi muscle is important for improving the current rejuvenation techniques or developing the new ones Nevertheless a study focusing on this topic has never been performed before simply because it is difficult and dangerous to perform needle biopsy on orbicularis oculi muscle
Detailed Description:
Hundreds of people came to our hospital requesting upper blepharoplasty every year Taking advantage of the discarded soft tissue in the blepharoplasty surgery samples of bilateral orbicularis oculi muscle can be easily obtained from patients of various ages This study is designed to objectively evaluate the age-related changes in human orbicularis oculi muscle by analyzing the composition of isoforms of myosin heavy chain MHC which is the predominant protein in skeletal muscle MHC isoforms exist in different types of muscle fibers and determines the maximum contraction speed of the muscle fiber Therefore The composition of MHC isoforms represents the composition of different types of muscle fiber
These muscle specimens are stored with liquid nitrogen right after surgery In the lab the specimens are sectioned to 40μm in thickness and soaked in special buffer solution After electrophoresis and staining bands of different types of MHC can be visualized The gel is scanned and processed with an image analysis system The density of each band can be determined and the ratio of the different types of MHC can be calculated
By dividing the subjects who had their orbicularis oculi muscle sampled during upper blepharoplasty operation into age groups of 20 30 40 50 60 and 70 years these MHC subtypes composition ratios can be compared among the different groups Therefore the age-related changes of the composition of different muscle types can be concluded
These muscle specimens are stored with liquid nitrogen right after surgery In the lab the specimens are sectioned to 40μm in thickness and soaked in special buffer solution After electrophoresis and staining bands of different types of MHC can be visualized The gel is scanned and processed with an image analysis system The density of each band can be determined and the ratio of the different types of MHC can be calculated
By dividing the subjects who had their orbicularis oculi muscle sampled during upper blepharoplasty operation into age groups of 20 30 40 50 60 and 70 years these MHC subtypes composition ratios can be compared among the different groups Therefore the age-related changes of the composition of different muscle types can be concluded
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None