Ignite Creation Date:
2024-05-05 @ 11:54 AM
Last Modification Date:
2024-10-26 @ 9:16 AM
Study NCT ID:
NCT00173407
Status:
WITHDRAWN
Last Update Posted:
2012-12-27
First Post:
2005-09-12
Brief Title:
PTEN and IGFBP-3 Correlation in Ovarian Carcinoma Invasion
Sponsor:
National Taiwan University Hospital
Organization:
National Taiwan University Hospital
Study Overview
Official Title:
Correlation of PTEN and IGFBP-3 in the Invasion of Ovarian Endometrioid Carcinoma
Status:
WITHDRAWN
Status Verified Date:
2012-12
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
We have identified insulin-like growth factor binding protein IGFBP-3 as an invasion suppressor gene in ovarian endometrioid carcinoma and showed association with lower cancer migration invasion and metastasis Recently a novel model of ovarian EC formation from endometriosis was reported and PTEN was found to be a major protein involved Inactivation of PTEN has been reported in some ovarian EC tumors and methylation was suggested as one of the major epigenetic changes This tumorigenesis model has lots of similarity to our established invasion model Therefore we plan to study the important of PTEN expression in ovarian EC and if inactivation of PTEN and IFGBP-3 is through methylation Furthermore by studying the signal transduction pathways using PTEN and IGFBP-3 transfection we plan to study the mutual interaction between PTEN and IGFBP-3 on the suppression of tumor invasion in ovarian EC
Detailed Description:
We have successfully established an ovarian cancer cell line OVTW-59 which was derived from an ovarian endometrioid carcinoma EC and have also established an ovarian EC invasion model By using cDNA microarray and quantitative reverse-transcriptase polymerase chain reaction we identified insulin-like growth factor binding protein IGFBP-3 as an invasion suppressor gene which were associated with lower cancer migration invasion and metastasis Clinically lower IGFBP-3 was found associated with significantly higher tumor grade advanced stage and poor survival in patients with EC tumors Furthermore we have proved IGFBP-3 expression correlated with lower Erk activation but with no effect on the activation of Akt All these two signal transduction proteins have crucial roles in cancer invasion Recently a novel model of ovarian EC formation from endometriosis was reported and PTEN was found to be a major protein involved Inactivation of PTEN has been reported in some ovarian EC tumors and methylation was suggested as one of the major epigenetic changes This tumorigenesis model has lots of similarity to our established invasion model Therefore we plan to study the important of PTEN expression in ovarian EC and if inactivation of PTEN and IFGBP-3 is through methylation Furthermore by studying the signal transduction pathways using PTEN and IGFBP-3 transfection we plan to study the mutual interaction between PTEN and IGFBP-3 on the suppression of tumor invasion in ovarian EC
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None