Ignite Creation Date:
2024-05-05 @ 11:55 AM
Last Modification Date:
2024-10-26 @ 9:16 AM
Study NCT ID:
NCT00177034
Status:
TERMINATED
Last Update Posted:
2012-07-10
First Post:
2005-09-12
Brief Title:
Examination Of Vitiligo Skin Samples Before and After UVB Treatment
Sponsor:
Mimi Cho MD
Organization:
University of Minnesota
Study Overview
Official Title:
Characterization of Keratinocyte Populations From Normal Perilesional and Lesional Skin in Vitiligo Patients by Laser Capture Microdissection and Gene Array Analysis Before and After Narrow Band UVB Treatment
Status:
TERMINATED
Status Verified Date:
2012-07
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
The keratinocytes of lesional skin of vitiligo compared to normal and perilesional skin will differentially express genes correlating with melanocyte death Narrow Band UVB should then hopefully reverse the pattern of gene expression back toward normal
Detailed Description:
Vitiligo is a disease caused by the disappearance of melanocytes in the epidermis The pathogenesis of vitiligo is multifactorial Theories include autoimmunity neural apoptosis and cytotoxicity The medical treatments for vitiligo are marginal with a 40-60 response rate which does not guarantee full repigmentation Understanding the pathogenesis of vitiligo will allow better targets for treatment
Previous analysis of skin biopsies have found several of the changes in gene expression occurred in immune regulation DNA replication and repair oncogenes signal transducers and transcription factors These results give us insight as to what happens in lesional vs nonlesional skin which contains the epidermis dermis fat blood vessels and immune cells
We have the technology today to study specific cell populations from tissue by the use of laser capture microdissection We propose to use this technique to study the keratinocyte population in vitiligo skin because past evidence suggests that they may play a role In addition the role keratinocytes in vitiligo may be important for treatment as this would be the ideal target for therapy Keratinocytes have been studied in vitiligo
The aim of the study is to isolate keratinocytes from normal skin perilesional and lesional skin in vitiligo patients by laser capture microdissection and characterize their gene expression profile by gene microarray analysis and to compare keratinocyte gene expression in normal perilesional and lesional vitiligo skin before and after narrow band UVB treatment
Previous analysis of skin biopsies have found several of the changes in gene expression occurred in immune regulation DNA replication and repair oncogenes signal transducers and transcription factors These results give us insight as to what happens in lesional vs nonlesional skin which contains the epidermis dermis fat blood vessels and immune cells
We have the technology today to study specific cell populations from tissue by the use of laser capture microdissection We propose to use this technique to study the keratinocyte population in vitiligo skin because past evidence suggests that they may play a role In addition the role keratinocytes in vitiligo may be important for treatment as this would be the ideal target for therapy Keratinocytes have been studied in vitiligo
The aim of the study is to isolate keratinocytes from normal skin perilesional and lesional skin in vitiligo patients by laser capture microdissection and characterize their gene expression profile by gene microarray analysis and to compare keratinocyte gene expression in normal perilesional and lesional vitiligo skin before and after narrow band UVB treatment
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None