Ignite Creation Date:
2024-05-05 @ 11:55 AM
Last Modification Date:
2024-10-26 @ 9:16 AM
Study NCT ID:
NCT00172588
Status:
UNKNOWN
Last Update Posted:
2005-11-28
First Post:
2005-09-12
Brief Title:
Evaluation of Endometrial Stromal Cell Apoptosis in Adenomyosis
Sponsor:
National Taiwan University Hospital
Organization:
National Taiwan University Hospital
Study Overview
Official Title:
None
Status:
UNKNOWN
Status Verified Date:
2004-12
Last Known Status:
RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Our previous results revealed that the expression of killer inhibitory receptors KIRs on NK cells was decreased in eutopic endometrium in women with adenomyosis It implies that the formation of adenomyosis might be due to abnormal endometrial tissues but not the aberrant local immunological dysfunction in myometrium In addition in vitro coculture of macrophages and endometrial stromal cells ESC increase the expression of IL-6 mRNA in ESC which might further enhance the proliferation of ESC and subsequently result in the formation of ectopic endometrial implants in adenomyosis Besides another possible mechanism is the abnormal apoptosis of ESC in adenomyosis In endometriosis apoptotic endometrial cells were found to be decreased which possibly accounts for the formation of endometriosis However there have not been reports investigating the apoptosis of ESC in adenomyosis
In this study we try to investigate apoptotic ESC with flow cytometry in women with and without adenomyosis Annexin V bcl-2 and caspase-3 were measured to represent the degree of apoptosis in ESC
In this study we try to investigate apoptotic ESC with flow cytometry in women with and without adenomyosis Annexin V bcl-2 and caspase-3 were measured to represent the degree of apoptosis in ESC
Detailed Description:
Eutopic endometrium was obtained and separated into single endometrial stromal cell ESC in women with adenomyosis study group and without adenomyosis control group
Annexin V-PE bcl-2-PE and caspase-3-PE are stained and flow cytometry is done to measure the degree of apoptosis in ESC On the other hand another half of ESC are cultured for 24h and also Annexin V-PE bcl-2-PE and caspase-3-PE are stained and flow cytometry is done
Annexin V-PE bcl-2-PE and caspase-3-PE are stained and flow cytometry is done to measure the degree of apoptosis in ESC On the other hand another half of ESC are cultured for 24h and also Annexin V-PE bcl-2-PE and caspase-3-PE are stained and flow cytometry is done
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None