Ignite Creation Date:
2024-05-06 @ 9:36 AM
Last Modification Date:
2024-10-26 @ 12:17 PM
Study NCT ID:
NCT03026361
Status:
COMPLETED
Last Update Posted:
2017-01-20
First Post:
2017-01-18
Brief Title:
Molecular Mechanisms of the Development of Precancerous and Cancerous Lesions of the Oral Cavity
Sponsor:
University of Zagreb
Organization:
University of Zagreb
Study Overview
Official Title:
Molecular Mechanisms of Precancerous and Cancerous Lesions of the Oral Cavity
Status:
COMPLETED
Status Verified Date:
2017-01
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
The aim of this study was to examine molecular alterations on the protein level in lesions of oral lichen planus OLP oral squamous cell carcinoma OSCC and healthy mucosa Global protein profiling methods based on liquid chromatography coupled to mass spectrometry were used with a special emphasis on evaluation of deregulated extracellular matrix molecules expression as well as on analyses of insulin-like growtg factor 2 IG2F and insulin-like growth factor 2 receptor IGFR2 expression in healthy mucosa OLP and OSCC tissues by comparative semiquantitative immunohistochemistry
Mass spectrometry based proteomics profiling of healthy mucosa OLP and OSCC tissues and accompanied histologically unaltered tissues respectively identified 55 extracellular matrix proteins Twenty among identified proteins were common to all groups of samples Statistically significant difference between final IGF2 and IGF2R IRS scores in favour to IGF2R may further corroborate the IG2FR antitumor role in OLP and OSCC where it acts as a negative regulator of IGF2 activity
Mass spectrometry based proteomics profiling of healthy mucosa OLP and OSCC tissues and accompanied histologically unaltered tissues respectively identified 55 extracellular matrix proteins Twenty among identified proteins were common to all groups of samples Statistically significant difference between final IGF2 and IGF2R IRS scores in favour to IGF2R may further corroborate the IG2FR antitumor role in OLP and OSCC where it acts as a negative regulator of IGF2 activity
Detailed Description:
Although OLP is categorised as a precancerous condition associated with a significantly increased risk of oral cancer molecular pathophysiology of OLP and its potential for malignant transformation in OSCC are poorly understood and remain controversial Development of new analytical methods enhances research in the field of malignant disorders Identification of novel biomarkers help in the diagnostic process pre-symptomatic interventions or prediction of treatment response Proteomics based on mass spectrometry enables analysis of novel putative biomarkers In this study proteomics was used to analyse in more details extracellular matrix ECM proteins and proteins related to ECM signalization which have a well-established role in malignant transformation and invasion of tumor cells
IGF2 and IGF2R are known biomarkers in cellular metabolismbut their role in oral precancerous lesions namely oral lichen planus OLP as well as in oral squamous cell carcinoma OSCC has not been explored so far The second aim of our study was to analyse IG2F and IGFR2 expression in oral lichen planus and oral squamous cell carcinoma tissues by comparative semiquantitative immunohistochemistry
IGF2 and IGF2R are known biomarkers in cellular metabolismbut their role in oral precancerous lesions namely oral lichen planus OLP as well as in oral squamous cell carcinoma OSCC has not been explored so far The second aim of our study was to analyse IG2F and IGFR2 expression in oral lichen planus and oral squamous cell carcinoma tissues by comparative semiquantitative immunohistochemistry
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None