Ignite Creation Date:
2024-05-06 @ 11:56 AM
Last Modification Date:
2024-10-26 @ 12:52 PM
Study NCT ID:
NCT03635294
Status:
COMPLETED
Last Update Posted:
2020-01-03
First Post:
2018-08-10
Brief Title:
Multi-Omics and IPSCs to Improve Diagnosis of Rare Intellectual Disabilities
Sponsor:
University Hospital Angers
Organization:
University Hospital Angers
Study Overview
Official Title:
Multi-Omics and IPSCs to Improve the Diagnosis of Rare Intellectual Disabilities
Status:
COMPLETED
Status Verified Date:
2024-08
Last Known Status:
RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
MIDRID
Brief Summary:
Background Genetic factors play a major role in intellectual disability ID but the underlying cause is not determined in many cases
This proposal is the continuation of the previous interregional project HUGODIMS the aim of which was to perform whole exome sequencing WES in 69 thoroughly selected simplex ID parent-child trios Thanks to HUGODIMS consortium the underlying genetic cause of ID was determined or highly suspected in 48 cases 695 and 7 novel ID genes were identified
Hypothesis Investigators hypothesize that an approach combining genomics transcriptomics metabolomics and morphological analyses performed on induced pluripotent stem cell iPSC-derived neural cells would improve diagnosis of ID The current proposal is therefore a proof-of concept project aiming at assessing the relevance and effectiveness of this multi-omics approach
Aims and Methods Ten individuals with ID recruited through HUGODIMS in whom WES have failed to identify pathogenic variants will be included
The workflow is the following
1 Whole genome sequencing WGS Nantes of these 10 negative trios
2 Bio-informatics analyses
3 In 3 WGS negative cases 3 positive controls bearing distinct mutations in CAMK2a a novel ID gene identified thanks to HUGODIMS and 3 healthy negative controls
1 Derivation of induced pluripotent stem cell iPSC-derived neural progenitors iPSC core facility at Nantes
2 Targeted and non-targeted metabolomics analyses performed on iPSC-derived neuronal cells Angers
3 RNA sequencing performed on the 9 cell lines Rennes
4 Morphological analyses of differentiated neuronal cell lines derived from 3 affected individuals and 3 positive controls bearing CMK2a mutations Tours
5 Integration and validation of data from multi-omics and morphological approaches
Expected results and impact Investigatrors expect that this approach combining multi-omics and iPSC will help to improve diagnosis and understanding of genetic ID of unknown cause
This proposal is the continuation of the previous interregional project HUGODIMS the aim of which was to perform whole exome sequencing WES in 69 thoroughly selected simplex ID parent-child trios Thanks to HUGODIMS consortium the underlying genetic cause of ID was determined or highly suspected in 48 cases 695 and 7 novel ID genes were identified
Hypothesis Investigators hypothesize that an approach combining genomics transcriptomics metabolomics and morphological analyses performed on induced pluripotent stem cell iPSC-derived neural cells would improve diagnosis of ID The current proposal is therefore a proof-of concept project aiming at assessing the relevance and effectiveness of this multi-omics approach
Aims and Methods Ten individuals with ID recruited through HUGODIMS in whom WES have failed to identify pathogenic variants will be included
The workflow is the following
1 Whole genome sequencing WGS Nantes of these 10 negative trios
2 Bio-informatics analyses
3 In 3 WGS negative cases 3 positive controls bearing distinct mutations in CAMK2a a novel ID gene identified thanks to HUGODIMS and 3 healthy negative controls
1 Derivation of induced pluripotent stem cell iPSC-derived neural progenitors iPSC core facility at Nantes
2 Targeted and non-targeted metabolomics analyses performed on iPSC-derived neuronal cells Angers
3 RNA sequencing performed on the 9 cell lines Rennes
4 Morphological analyses of differentiated neuronal cell lines derived from 3 affected individuals and 3 positive controls bearing CMK2a mutations Tours
5 Integration and validation of data from multi-omics and morphological approaches
Expected results and impact Investigatrors expect that this approach combining multi-omics and iPSC will help to improve diagnosis and understanding of genetic ID of unknown cause
Detailed Description:
None
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None