Ignite Creation Date:
2024-05-05 @ 4:49 PM
Last Modification Date:
2024-10-26 @ 9:25 AM
Study NCT ID:
NCT00327301
Status:
WITHDRAWN
Last Update Posted:
2015-03-10
First Post:
2006-05-17
Brief Title:
Cellular Proteome From Leukocytes of Glaucoma Patients in Comparison With Patients With Parkinsons Disease
Sponsor:
University Hospital Basel Switzerland
Organization:
University Hospital Basel Switzerland
Study Overview
Official Title:
Systematic Characterization of the Cellular Proteome From Human Leukocytes of Glaucoma Patients in Comparison With Patients With Parkinsons Disease
Status:
WITHDRAWN
Status Verified Date:
2015-03
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
was replaced by an other study
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Glaucoma is a worldwide leading cause of blindness The key feature of this ocular neuropathy is characterized by an excavating optic nerve head Loss of retinal ganglion cells is the final end point in blinding diseases of the optic nerve such as glaucoma It is known that neuronal cell death in glaucoma occurs by an apoptotic mechanism In earlier studies the investigators could demonstrate that the process of apoptosis is reflected in circulating leukocytes by different parameters like differential mRNA expression and an increased fragmentation of the DNA Such alterations point out a relationship between cellular stress and apoptotic events
Based on the results of mRNA-expression the investigators also expect alterations on the protein level
This study is therefore designed to characterize the proteome related to the proteins involved in cell death related pathways
Thus the expression pattern of several proteins in leukocytes from patients with primary open angle glaucoma will be analyzed by techniques like Western-blot and tandem mass spectrometry These samples will be compared with samples from healthy controls In addition they will also be compared with samples from patients with Parkinsons disease Since glaucoma is a neurodegenerative disease these patients will be included as positive controls in this study
Based on the results of mRNA-expression the investigators also expect alterations on the protein level
This study is therefore designed to characterize the proteome related to the proteins involved in cell death related pathways
Thus the expression pattern of several proteins in leukocytes from patients with primary open angle glaucoma will be analyzed by techniques like Western-blot and tandem mass spectrometry These samples will be compared with samples from healthy controls In addition they will also be compared with samples from patients with Parkinsons disease Since glaucoma is a neurodegenerative disease these patients will be included as positive controls in this study
Detailed Description:
Hypothesis
Differences in the proteome concerning cell death pathways of glaucoma patients correspond to the differences in the mRNA expression of these patients
Specific aims
Characterization of the cellular proteome from human leukocytes of glaucoma patients compared to healthy controls and patients with Parkinsons disease
Background
Glaucoma is a worldwide leading cause of blindness The key feature of this ocular neuropathy is characterized by an excavating optic nerve head Loss of retinal ganglion cells is the final end point in blinding diseases of the optic nerve such as glaucoma It is known that neuronal cell death in glaucoma occurs by an apoptotic mechanism In earlier studies we could demonstrate that this cell death is reflected in circulating leukocytes by different parameters like differential mRNA expression and an increased fragmentation of the DNA The differences in mRNA expression indicate a close relationship to cellular stress conditions and apoptotic events increased mRNA expression was detected for p53 20S proteasome alpha subunit ABC1 transporter p21WAF1CIP1 14-3-3 sigma factor MMP-9 and MMP-14 and TIMP-1
Based on the assumption that glaucoma patients may differ on the level of their expression for these mRNAs we expect that similar differences should exist at the protein level
This study is therefore designed to characterize the proteome related to the proteins involved in cell death related pathways
Differences in the proteome concerning cell death pathways of glaucoma patients correspond to the differences in the mRNA expression of these patients
Specific aims
Characterization of the cellular proteome from human leukocytes of glaucoma patients compared to healthy controls and patients with Parkinsons disease
Background
Glaucoma is a worldwide leading cause of blindness The key feature of this ocular neuropathy is characterized by an excavating optic nerve head Loss of retinal ganglion cells is the final end point in blinding diseases of the optic nerve such as glaucoma It is known that neuronal cell death in glaucoma occurs by an apoptotic mechanism In earlier studies we could demonstrate that this cell death is reflected in circulating leukocytes by different parameters like differential mRNA expression and an increased fragmentation of the DNA The differences in mRNA expression indicate a close relationship to cellular stress conditions and apoptotic events increased mRNA expression was detected for p53 20S proteasome alpha subunit ABC1 transporter p21WAF1CIP1 14-3-3 sigma factor MMP-9 and MMP-14 and TIMP-1
Based on the assumption that glaucoma patients may differ on the level of their expression for these mRNAs we expect that similar differences should exist at the protein level
This study is therefore designed to characterize the proteome related to the proteins involved in cell death related pathways
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None