Ignite Creation Date:
2024-05-06 @ 2:16 PM
Last Modification Date:
2024-10-26 @ 1:27 PM
Study NCT ID:
NCT04261595
Status:
COMPLETED
Last Update Posted:
2022-06-03
First Post:
2020-02-01
Brief Title:
Dates as a Functional Food for Autism
Sponsor:
National Research Centre Egypt
Organization:
National Research Centre Egypt
Study Overview
Official Title:
Evaluating Dates as a Functional Food for Autism Via Its Prebiotic Effect Modulation of Anti-Inflammatory and Anti-Oxidative Activity
Status:
COMPLETED
Status Verified Date:
2022-06
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Autism spectrum disorder ASD is a lifelong developmental disability that includes deficits in social communication and social interaction and restricted repetitive patterns of behavior interests or activities
The current treatment approaches for the management of autism are expensive showing unwanted adverse effects alter the genetic and metabolic pathways Many studies referred to the importance of probiotics minerals and antioxidant supplementation in the treatment of accompanied eating and feeding problems Meanwhile there are still controversial results about the effectiveness of dietary interventions for ASD like the gluten-freecasein-free diet and the ketogenic diet
Dates have been documented in the Holy Quran and proved in modern scientific literature to act as potent antioxidants and as an anti-inflammatory provide a suitable alternative therapy in various diseases cure
The medicinal therapeutic value of date fruits as nutritional probiotic and its implications in controlling autism through the anti-oxidant effect will be evaluated Moreover at which dose Dates fruit can exert its effect and whether this effect is sustained or not will be also evaluated Besides Dates effect on cognitive neurological parameters eating behaviors and gastrointestinal symptoms and anthropometric measures will be evaluated A non-randomized clinical study on 120 diagnosed autistic children aged 3-12 years in Cairo will be conducted Participants will be randomly assigned to one of three groups each with 40 children receiving one of three regimens the first group will receive 3 Dates per day for 3 months and the second group will receive 5 Dates per day for 3 months and the third group will receive nothing All groups will receive nutrition guidance behavioral modification and stimulation sessions Stools and blood samples will be collected before the study after 12 weeks of the intervention and then 12 weeks after completion of the intervention Evaluating Dates ability to decrease the oxidative stress will be done through comparing blood levels of three oxidative markers Malondialdehyde MDA glutathione peroxidase GPX1 and superoxide dismutase SOD in children with autism for the three proposed regimen The impact of the Dates fruit consumption on the microbiota of the autistic children both the pathogenic bacteria and probiotics will be done through traditional stool analysis and Real-time PCR before and after interventions
The current treatment approaches for the management of autism are expensive showing unwanted adverse effects alter the genetic and metabolic pathways Many studies referred to the importance of probiotics minerals and antioxidant supplementation in the treatment of accompanied eating and feeding problems Meanwhile there are still controversial results about the effectiveness of dietary interventions for ASD like the gluten-freecasein-free diet and the ketogenic diet
Dates have been documented in the Holy Quran and proved in modern scientific literature to act as potent antioxidants and as an anti-inflammatory provide a suitable alternative therapy in various diseases cure
The medicinal therapeutic value of date fruits as nutritional probiotic and its implications in controlling autism through the anti-oxidant effect will be evaluated Moreover at which dose Dates fruit can exert its effect and whether this effect is sustained or not will be also evaluated Besides Dates effect on cognitive neurological parameters eating behaviors and gastrointestinal symptoms and anthropometric measures will be evaluated A non-randomized clinical study on 120 diagnosed autistic children aged 3-12 years in Cairo will be conducted Participants will be randomly assigned to one of three groups each with 40 children receiving one of three regimens the first group will receive 3 Dates per day for 3 months and the second group will receive 5 Dates per day for 3 months and the third group will receive nothing All groups will receive nutrition guidance behavioral modification and stimulation sessions Stools and blood samples will be collected before the study after 12 weeks of the intervention and then 12 weeks after completion of the intervention Evaluating Dates ability to decrease the oxidative stress will be done through comparing blood levels of three oxidative markers Malondialdehyde MDA glutathione peroxidase GPX1 and superoxide dismutase SOD in children with autism for the three proposed regimen The impact of the Dates fruit consumption on the microbiota of the autistic children both the pathogenic bacteria and probiotics will be done through traditional stool analysis and Real-time PCR before and after interventions
Detailed Description:
ASD is progressively increasing the rate of autism was 34 per 1000 for children aged 3 to 10 years in the 1990s Meanwhile in 2006 the prevalence estimates are in the range of 65 to 66 per 1000 and recently 1 in 160 children have ASD Though classified as a neurodevelopmental condition ASD is often associated with multiple medical comorbidities and gastrointestinal GI problems GI disorders occurred in around 90 of ASD children with suggested possible role of the GI microflora in the severity of symptoms in autistic children
Children with ASD frequently have food selectivity which may have an effect on gastrointestinal symptoms The gut flora of autistic children is altered compared to healthy children and they have multiple nutritional deficiencies The microbiota that resides in the gut was claimed to be the cause behind the neurodevelopmental disorders occurring among the autistic children
Due to the frequent occurrence of gastrointestinal dysfunction in patients with ASD the use of probiotics may be an indication for adjuvant therapy Probiotics are living nonpathogenic microorganisms when administered in adequate amounts exert their beneficial effects resulting in modulation of the immune and inflammatory response The supplementation of probiotic bacteria to autistic children can lead to changes in the microbiota and it considers a useful new therapeutic tool to normalize gut microbiota restore the function of the epithelial barrier resulting in reliving of the gastrointestinal system disorders reduce inflammation and potentially improve the behavioral symptoms in autistic children
Furthermore children with autism have deficient antioxidant defense Mechanisms They are vulnerable to oxidative stress which is detected by elevated lipid peroxidation especially at younger children Oxidative stress may play a role in the development and clinical manifestations of autism resulting in loss of acquired language skills in autism Meguid and coworkers highlighted that autistic children might benefit from antioxidant supplementation coupled with polyunsaturated fatty acids Early assessment and intervention of antioxidant status may decrease the oxidative stress before inducing more irreversible brain damage
In Islam herbs and its constituents have important value in diet and treatment of various types of diseases The Prophet Mohammed Peace Be Upon Him used various herbs and recommended various medicinal plants for the cure of various diseases
Dates and their constituents show a role in disease prevention through anti-oxidant anti-inflammatory anti-bacterial activity Dates are considered a staple food item in the Middle East and North Africa and are also imported in Europe the UK and the USA
Dates contain relatively high levels of polyphenols and insoluble fiber It has shown that whole date fruit and polyphenols extracted from them selectively stimulates Bifidobacterium spp and Bacteroides along with the total bacterial counts in fecal batch culture models increasing small chain fatty acid SCFA production concurrently In addition promising changes in DNA damage were observed through the anti-proliferative actions of both polyphenol extracts and whole date extracts
Antioxidant activity of Dates is recognized due to the wide range of phenolic compounds present in Dates including p-coumaric ferulic and sinapic acids flavonoids and procyanidins
Dates fruit is considered as a rich source of minerals and sugars its various constituents like phytochemicals carotenoids steroids and flavonoids are screened for numerous medicine Rahmani and his colleagues illustrate all the therapeutic functions of the Dates
Keeping all information in hands about Dates therapeutic effect in the management of the disease via its anti-oxidant anti-inflammatory effect and for the sake of autistic children It is worthy to point and essential to evaluate Dates as a good remedy for autistic children because it is inexpensive and easy to access
Objectives
The overall objective is to discover functional food that is effective to control the comorbidities accompanied by autism development and to limit its progression This food should be safe inexpensive and affordable easy to access without any complications
The medicinal therapeutic value of Dates fruit as nutritional prebiotic and its implications in controlling autism through anti-oxidant and anti-inflammatory effects will be evaluated
Specific Objectives
The aim of this interventional study is to
1 Evaluate Dates effect on cognitive development
2 Evaluate Dates effect on neurological parameters and severity of autism
3 Evaluate Dates effect on eating behaviors and diet quality
4 Evaluate Dates effect on gastrointestinal symptoms and the microbiota
5 Evaluate Dates ability to decrease the oxidative stress before inducing more irreversible brain damage through comparing levels of three oxidative markers Malondialdehyde MDA glutathione peroxidase GPX1 and superoxide dismutase SOD in children with autism for the three proposed regimen
6 Evaluate at which dose Dates fruit can exert its effect as a new therapeutic regimen and its persistence over time
Phases of the study and tools
This study will be conducted as three phases that will be done in a simultaneous way
The first phase will include patient recruitment and baseline assessment before Dates fruit intake
1 Applying the confirmatory diagnostic tools for autistic patients aged 3 -12 years Children with suspected ASD could be then assessed according to either Diagnostic and statistical manual of mental disorders 5th edition DSM-V criteria and CARS
An autism screening tool must meet all three primary areas defined by the DSM-V description for the autistic disorder to qualify for a positive rating from First Signs
A A total of six or more items from 1 2 and 3 with at least two from 1 and one each from 2 and 3
1 Qualitative impairment in social interaction as manifested by at least two of the following
1 Marked impairment in the use of multiple nonverbal behaviors such as eye-to-eye gaze facial expression body postures and gestures to regulate social interaction
2 Failure to develop peer relationships appropriate to the developmental level
3 A lack of spontaneous seeking to share enjoyment interests or achievements with other people eg by a lack of showing bringing or pointing out objects of interest
4 Lack of social or emotional reciprocity
2 Qualitative impairments in communication as manifested by at least one of the following
1 Delay in or total lack of the development of spoken language not accompanied by an attempt to compensate through alternative modes of communication such as gesture or mime
2 In individuals with adequate speech marked impairment in the ability to initiate or sustain a conversation with others
3 Stereotyped and repetitive use of language or idiosyncratic language
4 Lack of varied spontaneous make-believe play or social imitative play
3 Restricted repetitive and stereotyped patterns of behavior interests and activities as manifested by at least one of the following
1 Encompassing preoccupation with one or more stereotyped and restricted patterns of interest that is abnormal either in intensity or focus
2 Apparently inflexible adherence to specific nonfunctional routines or rituals
3 Stereotyped and repetitive motor mannerisms eg hand or finger flapping or twisting or complex whole-body movements
4 Persistent pre-co-occupation with parts of objects B Delays or abnormal functioning in at least one of the following areas with onset prior to age 3 years 1 social interaction 2 language as used in social communication or 3 symbolic or imaginative play
The Instruments of the investigation will also include socio-demographic assessment questionnaire including suggested history of risk factors Demographics Medication exposure Medical conditions Pesticides Diet Home environmental exposures Health behaviorslifestyle Mental conditionhistorysymptoms Vaccine history Personal product use Anthropometrics Medical procedures Occupational history In addition the questionnaire will focus on socioeconomic indicators as well as prenatal and perinatal and neonatal risk factors such as the timing of conception weight gain during pregnancy parental age and inter-pregnancy interval
2 Assessment of the sociodemographic characteristics through detailed history taking pregnancy history peri-natal history and other risk factors
3 Neurological assessment through thorough clinical examination
4 Assessing functional gastrointestinal behavior disorders FGID Assessing changes in the GI symptoms which may be constipation diarrhea gaseousness bloating gastroesophageal reflux abdominal pain and vomiting but constipation is the more common and the most reported GI symptoms
5 Detailed nutritional and dietary assessment with anthropometric measurements Anthropometric measurements
1 Weight in kg by Body fat analyzer
2 Height in cm using Stadiometer
3 BMI weightHeight2 in metercalculated
4 Waist circumference in cm measuring tapes
5 Hip circumference in cm measuring tapes
6 Waist Hip ratio calculated
7 measuring body composition analyzer Bioelectrical impedance for autistic children aged seven years and older measure fat mass fat-free mass fat water content Basal metabolic rate BMR
6 Behavioral Assessment the interview questions will cover eight content areas
1 The subjects background including family education previous diagnoses and medications
2 Overview of the subjects behavior
3 Early development and developmental milestones
4 Language acquisition and loss of language or other skills
5 Current functioning in regard to language and communication
6 Social development and play
7 Interests and behaviors
8 Clinically relevant behaviors such as aggression self-injury and possible epileptic features
Mental Development Psychomotor Development and Behavior Rating will be assessed by using the Bailey Scales of Infant and Toddler Development the cognitive language and motor abilities will be assessed as well as social-emotional and adaptive behavior Mental and psychomotor developmental quotients and mental age will be scored as well as the behavioral profile The Bailey Screening Test is an Observation Checklist for 5 domains of development cognitive language motor social-emotional adaptive behavior
The questionnaire will be directed for the mothers of all confirmed cases with ASD enrolled in the study
Second phase Intervention phase
Special emphasis will be directed towards focusing on treating accompanied neurological manifestations Third phase during this phase the outcome of the intervention on the stool microbiota and on the serum antioxidant markers will be evaluated twice once after completing the intervention and 3 months later
Children with ASD frequently have food selectivity which may have an effect on gastrointestinal symptoms The gut flora of autistic children is altered compared to healthy children and they have multiple nutritional deficiencies The microbiota that resides in the gut was claimed to be the cause behind the neurodevelopmental disorders occurring among the autistic children
Due to the frequent occurrence of gastrointestinal dysfunction in patients with ASD the use of probiotics may be an indication for adjuvant therapy Probiotics are living nonpathogenic microorganisms when administered in adequate amounts exert their beneficial effects resulting in modulation of the immune and inflammatory response The supplementation of probiotic bacteria to autistic children can lead to changes in the microbiota and it considers a useful new therapeutic tool to normalize gut microbiota restore the function of the epithelial barrier resulting in reliving of the gastrointestinal system disorders reduce inflammation and potentially improve the behavioral symptoms in autistic children
Furthermore children with autism have deficient antioxidant defense Mechanisms They are vulnerable to oxidative stress which is detected by elevated lipid peroxidation especially at younger children Oxidative stress may play a role in the development and clinical manifestations of autism resulting in loss of acquired language skills in autism Meguid and coworkers highlighted that autistic children might benefit from antioxidant supplementation coupled with polyunsaturated fatty acids Early assessment and intervention of antioxidant status may decrease the oxidative stress before inducing more irreversible brain damage
In Islam herbs and its constituents have important value in diet and treatment of various types of diseases The Prophet Mohammed Peace Be Upon Him used various herbs and recommended various medicinal plants for the cure of various diseases
Dates and their constituents show a role in disease prevention through anti-oxidant anti-inflammatory anti-bacterial activity Dates are considered a staple food item in the Middle East and North Africa and are also imported in Europe the UK and the USA
Dates contain relatively high levels of polyphenols and insoluble fiber It has shown that whole date fruit and polyphenols extracted from them selectively stimulates Bifidobacterium spp and Bacteroides along with the total bacterial counts in fecal batch culture models increasing small chain fatty acid SCFA production concurrently In addition promising changes in DNA damage were observed through the anti-proliferative actions of both polyphenol extracts and whole date extracts
Antioxidant activity of Dates is recognized due to the wide range of phenolic compounds present in Dates including p-coumaric ferulic and sinapic acids flavonoids and procyanidins
Dates fruit is considered as a rich source of minerals and sugars its various constituents like phytochemicals carotenoids steroids and flavonoids are screened for numerous medicine Rahmani and his colleagues illustrate all the therapeutic functions of the Dates
Keeping all information in hands about Dates therapeutic effect in the management of the disease via its anti-oxidant anti-inflammatory effect and for the sake of autistic children It is worthy to point and essential to evaluate Dates as a good remedy for autistic children because it is inexpensive and easy to access
Objectives
The overall objective is to discover functional food that is effective to control the comorbidities accompanied by autism development and to limit its progression This food should be safe inexpensive and affordable easy to access without any complications
The medicinal therapeutic value of Dates fruit as nutritional prebiotic and its implications in controlling autism through anti-oxidant and anti-inflammatory effects will be evaluated
Specific Objectives
The aim of this interventional study is to
1 Evaluate Dates effect on cognitive development
2 Evaluate Dates effect on neurological parameters and severity of autism
3 Evaluate Dates effect on eating behaviors and diet quality
4 Evaluate Dates effect on gastrointestinal symptoms and the microbiota
5 Evaluate Dates ability to decrease the oxidative stress before inducing more irreversible brain damage through comparing levels of three oxidative markers Malondialdehyde MDA glutathione peroxidase GPX1 and superoxide dismutase SOD in children with autism for the three proposed regimen
6 Evaluate at which dose Dates fruit can exert its effect as a new therapeutic regimen and its persistence over time
Phases of the study and tools
This study will be conducted as three phases that will be done in a simultaneous way
The first phase will include patient recruitment and baseline assessment before Dates fruit intake
1 Applying the confirmatory diagnostic tools for autistic patients aged 3 -12 years Children with suspected ASD could be then assessed according to either Diagnostic and statistical manual of mental disorders 5th edition DSM-V criteria and CARS
An autism screening tool must meet all three primary areas defined by the DSM-V description for the autistic disorder to qualify for a positive rating from First Signs
A A total of six or more items from 1 2 and 3 with at least two from 1 and one each from 2 and 3
1 Qualitative impairment in social interaction as manifested by at least two of the following
1 Marked impairment in the use of multiple nonverbal behaviors such as eye-to-eye gaze facial expression body postures and gestures to regulate social interaction
2 Failure to develop peer relationships appropriate to the developmental level
3 A lack of spontaneous seeking to share enjoyment interests or achievements with other people eg by a lack of showing bringing or pointing out objects of interest
4 Lack of social or emotional reciprocity
2 Qualitative impairments in communication as manifested by at least one of the following
1 Delay in or total lack of the development of spoken language not accompanied by an attempt to compensate through alternative modes of communication such as gesture or mime
2 In individuals with adequate speech marked impairment in the ability to initiate or sustain a conversation with others
3 Stereotyped and repetitive use of language or idiosyncratic language
4 Lack of varied spontaneous make-believe play or social imitative play
3 Restricted repetitive and stereotyped patterns of behavior interests and activities as manifested by at least one of the following
1 Encompassing preoccupation with one or more stereotyped and restricted patterns of interest that is abnormal either in intensity or focus
2 Apparently inflexible adherence to specific nonfunctional routines or rituals
3 Stereotyped and repetitive motor mannerisms eg hand or finger flapping or twisting or complex whole-body movements
4 Persistent pre-co-occupation with parts of objects B Delays or abnormal functioning in at least one of the following areas with onset prior to age 3 years 1 social interaction 2 language as used in social communication or 3 symbolic or imaginative play
The Instruments of the investigation will also include socio-demographic assessment questionnaire including suggested history of risk factors Demographics Medication exposure Medical conditions Pesticides Diet Home environmental exposures Health behaviorslifestyle Mental conditionhistorysymptoms Vaccine history Personal product use Anthropometrics Medical procedures Occupational history In addition the questionnaire will focus on socioeconomic indicators as well as prenatal and perinatal and neonatal risk factors such as the timing of conception weight gain during pregnancy parental age and inter-pregnancy interval
2 Assessment of the sociodemographic characteristics through detailed history taking pregnancy history peri-natal history and other risk factors
3 Neurological assessment through thorough clinical examination
4 Assessing functional gastrointestinal behavior disorders FGID Assessing changes in the GI symptoms which may be constipation diarrhea gaseousness bloating gastroesophageal reflux abdominal pain and vomiting but constipation is the more common and the most reported GI symptoms
5 Detailed nutritional and dietary assessment with anthropometric measurements Anthropometric measurements
1 Weight in kg by Body fat analyzer
2 Height in cm using Stadiometer
3 BMI weightHeight2 in metercalculated
4 Waist circumference in cm measuring tapes
5 Hip circumference in cm measuring tapes
6 Waist Hip ratio calculated
7 measuring body composition analyzer Bioelectrical impedance for autistic children aged seven years and older measure fat mass fat-free mass fat water content Basal metabolic rate BMR
6 Behavioral Assessment the interview questions will cover eight content areas
1 The subjects background including family education previous diagnoses and medications
2 Overview of the subjects behavior
3 Early development and developmental milestones
4 Language acquisition and loss of language or other skills
5 Current functioning in regard to language and communication
6 Social development and play
7 Interests and behaviors
8 Clinically relevant behaviors such as aggression self-injury and possible epileptic features
Mental Development Psychomotor Development and Behavior Rating will be assessed by using the Bailey Scales of Infant and Toddler Development the cognitive language and motor abilities will be assessed as well as social-emotional and adaptive behavior Mental and psychomotor developmental quotients and mental age will be scored as well as the behavioral profile The Bailey Screening Test is an Observation Checklist for 5 domains of development cognitive language motor social-emotional adaptive behavior
The questionnaire will be directed for the mothers of all confirmed cases with ASD enrolled in the study
Second phase Intervention phase
Special emphasis will be directed towards focusing on treating accompanied neurological manifestations Third phase during this phase the outcome of the intervention on the stool microbiota and on the serum antioxidant markers will be evaluated twice once after completing the intervention and 3 months later
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None