Ignite Creation Date:
2024-05-06 @ 4:17 PM
Last Modification Date:
2024-10-26 @ 2:07 PM
Study NCT ID:
NCT04937855
Status:
UNKNOWN
Last Update Posted:
2021-06-24
First Post:
2020-01-11
Brief Title:
The Mechanism of lncRNA NEAT1 in Alleviating Acute Respiratory Distress Syndrome Through miR-27b Regulated Nrf2 Pathway
Sponsor:
Beijing Anzhen Hospital
Organization:
Beijing Anzhen Hospital
Study Overview
Official Title:
The Mechanism of lncRNA NEAT1 in Alleviating Acute Respiratory Distress Syndrome Through miR-27b Regulated Nrf2 Pathway
Status:
UNKNOWN
Status Verified Date:
2021-06
Last Known Status:
ENROLLING_BY_INVITATION
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
The acute respiratory distress syndrome formerly known as the acute lung injury ARDSALI is a critical illness with high mortality due to the lack of effective treatment The pathogenesis of ARDSALI has not been fully elucidated Nuclear factor E2-related factor 2 Nrf2 plays a key role in regulating lung inflammation and oxidative stress which are closely related to lung injury in ARDSALI but its regulatory mechanism remains unclear The investigators provious study shown that microRNA-27b miR-27b downregulated Nrf2 to aggravate lung inflammation and histological injury Furthermore in lipopolysaccharide LPS-induced cell J774A1 inflammation model miR-27b was upregulated while the long non-coding RNA lncRNA NEAT1 was downregulated the putative binding sites of lncRNA NEAT1 and miR-27b were successfully predicted by bioinformatics approach Thus the investigators propose that NEAT1 plays as a competing endogenous RNA ceRNA to adsorb miR-27b and liberate Nrf2 therefore to attenuate lung inflammation and related lung injury in ARDSALI This project aims to explore the role of the lncRNA NEAT1 mir-27b Nrf2 signal axis in the development and treatment of ARDSALI in patients as well as in LPS-induced ALI animal and cell models by using bioinformatics molecular biology histomorphology and clinical phenotype approaches and to clarify the new mechanism in ARDSALI development and to provide new therapeutic targets
Detailed Description:
Collect blood and BALF from 400 ARDS patients at different time at check-in 24 48 and 72 h after check-in the hospital and 25 gender and age matching healthy controls Use RT-PCR to detect the expression of lncRNA NEAT1miR-27b and Nrf2 in blood and BALF of ARDS patients and health controls The expressions of inflammatory and oxidative stress associated factors NLRP3NF-κB-P65 p-P65IκBp-IκBHO-1NQO1caspase-1IL-1βIL-6IL-18TNF-α will be detected by western blotELISA and RT-PCR Moreover flow cytometry will be adopted to measure the numbers and kinds of cells in BALF Then analyze the differences of the expressions of lncRNA NEAT1miR-27b and Nrf2 in the groups To explore the correlation of expressions of lncRNA NEAT1miR-27b and Nrf2 with inflammation and oxidative stress in the groups Finally to declare the relative of lncRNA NEAT1miR-27b and Nrf2 with the time of mechanical ventilation severity and mortality in 28 days of ARDS patients
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None