Ignite Creation Date:
2024-05-06 @ 5:53 PM
Last Modification Date:
2024-10-26 @ 2:37 PM
Study NCT ID:
NCT05460312
Status:
NOT_YET_RECRUITING
Last Update Posted:
2023-01-26
First Post:
2022-07-12
Brief Title:
Protein Kinase A in Prostate Cancer Tissue
Sponsor:
Ziv Hospital
Organization:
Ziv Hospital
Study Overview
Official Title:
Assessment of the Expression and Activity of Protein Kinase A in Prostate Cancer Tissue
Status:
NOT_YET_RECRUITING
Status Verified Date:
2023-01
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Prostate cancer is the most common malignancy in men documented risk factors of prostate cancer are age ethnicity various genomic mutations and family history of prostate cancer The cellular mechanisms of malignant transformation are numerous and not completely understood A possible mechanism is induction of an inflammation resulting in cellular atypia and pre-malignant changes in the affected tissue by inducing a pro-inflammatory response or changes in extra cellular matrix Protein Kinase A PKA is a key stone enzyme in various intra-cellular processes Various infections inflammations and malignancies were proved to impact PKA activity The research hypothesis is that prostate cancer tissue will show a unique profile of PKA activity regulation and intracellular distribution
Detailed Description:
The study will be conducted among men referred to prostate biopsy due to clinical suspicion of malignancy 100 consecutive patients will undergo prostate biopsies Biopsies will be taken from both lobes of the prostate standard protocol and additional biopsies will be taken according to MRI findings Fusion biopsies if relevant One additional biopsy will be taken from each lobe of the prostate and immediately freezed in -80 Celsius degrees Routine biopsies will be fixed in formaldehyde and embedded in paraffin and will undergo routine pathological analysis and staining
Following pathological analysis paraffin slides will be undergo immunohistochemically staining for PKA comparing cancerous tissue to normal surrounding tissue the fresh frozen tissue will undergo western blot analysis for quantitative expression of PKA and its sub units
Following pathological analysis paraffin slides will be undergo immunohistochemically staining for PKA comparing cancerous tissue to normal surrounding tissue the fresh frozen tissue will undergo western blot analysis for quantitative expression of PKA and its sub units
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None