Ignite Creation Date:
2024-05-06 @ 6:48 PM
Last Modification Date:
2024-10-26 @ 2:55 PM
Study NCT ID:
NCT05789888
Status:
COMPLETED
Last Update Posted:
2023-03-29
First Post:
2023-03-08
Brief Title:
Genes Variability in Obesity and Normal Body Weight Patients
Sponsor:
Medical University of Bialystok
Organization:
Medical University of Bialystok
Study Overview
Official Title:
Evaluation of the Influence of SNP Variability of Stearoyl-CoA Desaturase SCD Gene of Nuclear DNA and MT-ATP6 Gene of Mitochondrial DNA and Environmental Factors on Phenotypic Variability of Visceral and Subcutaneous Adipose Tissue
Status:
COMPLETED
Status Verified Date:
2023-02
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
The goal of this clinical trial is to learn about variability of gene SCD and MT-ATP6 in describe participant population and health conditions The main questions it aims to answer are
whether there is variation in genes between obese and normal weight individuals
Participants will get body weight and high was assessed using the medical scale and stadiometer then basis on obtained results the Body Mass Index BMI will be calculated Next participants get determine the body composition and body composition analysis by bioelectrical impedance method to determined Visceral Adipose Tissue VAT in cm2 and Subcutaneous Adipose Tissue SAT in cm2 and and the VATSAT ratio was also determined Then order to examine the sequence of the genes swab will take from the oral cavity cheeks and palate using sterile swab The research was carried out at the Department of Dietetics and Clinical Nutrition Medical University of Bialystok
whether there is variation in genes between obese and normal weight individuals
Participants will get body weight and high was assessed using the medical scale and stadiometer then basis on obtained results the Body Mass Index BMI will be calculated Next participants get determine the body composition and body composition analysis by bioelectrical impedance method to determined Visceral Adipose Tissue VAT in cm2 and Subcutaneous Adipose Tissue SAT in cm2 and and the VATSAT ratio was also determined Then order to examine the sequence of the genes swab will take from the oral cavity cheeks and palate using sterile swab The research was carried out at the Department of Dietetics and Clinical Nutrition Medical University of Bialystok
Detailed Description:
The approved of the Bioethics Committee of the Medical University No APK0024072020 was obtained for the study 116 people meeting the inclusion criteria were included in the intervention and observational study The research was carried out at the Department of Dietetics and Clinical Nutrition at the Faculty of Health Sciences of the Medical University of Bialystok
The study took place from the April to the September 2021 The analysis were performed in the morning fasting without intensive physical ac-tivity Body weight and high was assessed using the medical scale and stadiometer with an accuracy of 001 kg for the body weight and 05 cm for the body high Then basis on obtained results the Body Mass Index BMI was calculatedBasis on the obtained results the participants were divided into the study group n75 and control group n41 The study group consisted 46 women and 29 men with primary obesity BMI300-399 kgm2 The control group consisted 31 women and 10 men with normal body weight BMI185-249 kgm2
In order to determine the body composition of participants a body composition analysis by bioelectrical impedance method was conducted which enabled the assessment of the following parameters total body fat mass kg total percentage of body fat total percentage of free fat mass The area of adipose tissue in the transverse section to the abdomen was also deter-mined Visceral Adipose Tissue VAT in cm2 and Subcutaneous Adipose Tissue SAT in cm2 and and the VATSAT ratio was also determined To determine the TOFI phenotype of abdominal fat distribution VATSAT ratio was used- above 1 for men and above 09 for women
Next swab from the oral cavity was took To isolate DNA from the swabs was used ready kit from AA Biotechnology After it we did the PCR reactions with the appropriate primers and clean up after PCR reaction was did Next we prepared the samples for Sanger sequencing
When we get the sequences we used the genetics programs Bioedit Chromas DnaSP Arlequin Mega Genepop to get number of haplotypes genes variability F statistics haplotype diversity haplotypes network and genetic tree of haplotypes
The study took place from the April to the September 2021 The analysis were performed in the morning fasting without intensive physical ac-tivity Body weight and high was assessed using the medical scale and stadiometer with an accuracy of 001 kg for the body weight and 05 cm for the body high Then basis on obtained results the Body Mass Index BMI was calculatedBasis on the obtained results the participants were divided into the study group n75 and control group n41 The study group consisted 46 women and 29 men with primary obesity BMI300-399 kgm2 The control group consisted 31 women and 10 men with normal body weight BMI185-249 kgm2
In order to determine the body composition of participants a body composition analysis by bioelectrical impedance method was conducted which enabled the assessment of the following parameters total body fat mass kg total percentage of body fat total percentage of free fat mass The area of adipose tissue in the transverse section to the abdomen was also deter-mined Visceral Adipose Tissue VAT in cm2 and Subcutaneous Adipose Tissue SAT in cm2 and and the VATSAT ratio was also determined To determine the TOFI phenotype of abdominal fat distribution VATSAT ratio was used- above 1 for men and above 09 for women
Next swab from the oral cavity was took To isolate DNA from the swabs was used ready kit from AA Biotechnology After it we did the PCR reactions with the appropriate primers and clean up after PCR reaction was did Next we prepared the samples for Sanger sequencing
When we get the sequences we used the genetics programs Bioedit Chromas DnaSP Arlequin Mega Genepop to get number of haplotypes genes variability F statistics haplotype diversity haplotypes network and genetic tree of haplotypes
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None