Ignite Creation Date:
2024-05-05 @ 11:21 AM
Last Modification Date:
2024-10-26 @ 9:05 AM
Study NCT ID:
NCT00005364
Status:
COMPLETED
Last Update Posted:
2016-05-13
First Post:
2000-05-25
Brief Title:
Genetic Epidemiology of CHD Risk Factors in Blacks
Sponsor:
National Heart Lung and Blood Institute NHLBI
Organization:
National Heart Lung and Blood Institute NHLBI
Study Overview
Official Title:
None
Status:
COMPLETED
Status Verified Date:
2000-06
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
To determine the genetic epidemiology of coronary heart disease CHD risk factors in Blacks
Detailed Description:
DESIGN NARRATIVE
The study used two well defined African populations from Nigeria to determine the contribution of 14 polymorphic genes involved in lipid metabolism in determining quantitative lipoprotein-lipid and apolipoprotein levels as these are major risk factors for coronary heart disease The study also investigated how differences in life styles and dietary habits modulate the genetic contribution of these quantitative risk factors Phenotypes in the gene products of APOs A-IV C-II D H J and a were determined by IEF and SDSimmunoblotting Genotypes at the BE AI-CIII- AIV cluster lipoprotein lipase hepatic lipase LDL-receptor and cholesterol ester transfer protein were determined by polymerase chain reaction protocols The quantitative levels of total cholesterol HDL-cholesterol and its subfractions HDL2- and HDL3-cholesterol triglycerides and LPa were used in the genetic analyses Estimates of the effect of alleles at each of the candidate loci on quantitative lipoprotein-lipid levels employed the measured genotype approach The effect of multisite haplotypes for RFLP at various loci where applicable was also estimated using the same method The interaction of alleles at various independent loci in determining quantitative lipoprotein-lipid and apolipoprotein levels was estimated In addition to the measurements of environmental effects measurements were also made of the genotype-environmental interaction in determining quantitative risk factor traits
The study completion date listed in this record was obtained from the End Date entered in the Protocol Registration and Results System PRS record
The study used two well defined African populations from Nigeria to determine the contribution of 14 polymorphic genes involved in lipid metabolism in determining quantitative lipoprotein-lipid and apolipoprotein levels as these are major risk factors for coronary heart disease The study also investigated how differences in life styles and dietary habits modulate the genetic contribution of these quantitative risk factors Phenotypes in the gene products of APOs A-IV C-II D H J and a were determined by IEF and SDSimmunoblotting Genotypes at the BE AI-CIII- AIV cluster lipoprotein lipase hepatic lipase LDL-receptor and cholesterol ester transfer protein were determined by polymerase chain reaction protocols The quantitative levels of total cholesterol HDL-cholesterol and its subfractions HDL2- and HDL3-cholesterol triglycerides and LPa were used in the genetic analyses Estimates of the effect of alleles at each of the candidate loci on quantitative lipoprotein-lipid levels employed the measured genotype approach The effect of multisite haplotypes for RFLP at various loci where applicable was also estimated using the same method The interaction of alleles at various independent loci in determining quantitative lipoprotein-lipid and apolipoprotein levels was estimated In addition to the measurements of environmental effects measurements were also made of the genotype-environmental interaction in determining quantitative risk factor traits
The study completion date listed in this record was obtained from the End Date entered in the Protocol Registration and Results System PRS record
Study Oversight
Has Oversight DMC:
Is a FDA Regulated Drug?:
Is a FDA Regulated Device?:
Is an Unapproved Device?:
Is a PPSD?:
Is a US Export?:
Is an FDA AA801 Violation?:
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| R01HL049074 | NIH | None | httpsreporternihgovquickSearchR01HL049074 |