Ignite Creation Date:
2024-06-16 @ 11:48 AM
Last Modification Date:
2024-10-26 @ 3:30 PM
Study NCT ID:
NCT06427408
Status:
NOT_YET_RECRUITING
Last Update Posted:
2024-05-23
First Post:
2024-05-16
Brief Title:
Longitudinal Study of Gene Expression in the Human Placenta During First-trimester and Full-term Pregnancy at the Single Cell Level
Sponsor:
Assistance Publique - Hôpitaux de Paris
Organization:
Assistance Publique - Hôpitaux de Paris
Study Overview
Official Title:
Cellular Transcriptomic Trajectory of the Human Placenta During Pregnancy
Status:
NOT_YET_RECRUITING
Status Verified Date:
2024-05
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
VilliRNAseq
Brief Summary:
The single nucleus RNA-seq approach allows the placental transcriptome to be analyzed from nuclei thus preserving the integrity of placental syncytium This approach is feasible on small fragments of villi and offers for the first time the possibility to consider the characterization of gene expression within the structural unit of the human placenta during pregnancy at the 1st trimester and childbirth
Detailed Description:
Chorionic villus biopsies are routinely performed between 11SA and 15SA at the Fetal Medicine Unit of the Port-Royal Maternity Hospital CPDPN Cochin after the patient has signed an information and consent form for the genetic analysis
The patient will be asked to participate to the VilliRNAseq project and will sign the consent form of the study After the cytogeneticist observation a villi fragment will be snap-frozen in liquid nitrogen and stored at -80C until use Each patient sampled in the first trimester is followed until the end of the pregnancy At delivery cesarean section or vaginal delivery a placental sample will be snap-frozen in liquid nitrogen and stored at -80C until use
Isolation sorting and encapsidation of nuclei RNA libraries preparation and sequencing will be carried out using 10x Genomics technology State-of-the-art computational analyses to decipher trajectories and cell-to-cell communications
The patient will be asked to participate to the VilliRNAseq project and will sign the consent form of the study After the cytogeneticist observation a villi fragment will be snap-frozen in liquid nitrogen and stored at -80C until use Each patient sampled in the first trimester is followed until the end of the pregnancy At delivery cesarean section or vaginal delivery a placental sample will be snap-frozen in liquid nitrogen and stored at -80C until use
Isolation sorting and encapsidation of nuclei RNA libraries preparation and sequencing will be carried out using 10x Genomics technology State-of-the-art computational analyses to decipher trajectories and cell-to-cell communications
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| 2022-A02452-41 | OTHER | France Ministry of Health | None |