Ignite Creation Date:
2024-07-17 @ 11:27 AM
Last Modification Date:
2024-10-26 @ 3:33 PM
Study NCT ID:
NCT06484101
Status:
COMPLETED
Last Update Posted:
2024-07-03
First Post:
2024-06-18
Brief Title:
Functional Characterization of CD8 T Cells After Supramaximal Exercise
Sponsor:
Karolinska Institutet
Organization:
Karolinska Institutet
Study Overview
Official Title:
Effekten av högintensiv träning på Vita Blodkroppar Med Speciellt Fokus på Funktion Kopplad Till Att bekämpa Cancerceller
Status:
COMPLETED
Status Verified Date:
2024-06
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Healthy human subjects perform supramaximal exercise with venous blood samples obtained before and up to 60 minutes after the exercise bout
Detailed Description:
Healthy human subjects perform 3 x 30 seconds of supramaximal exercise Venous blood samples were obtained before and up to 60 minutes after the exercise bout The blood was used for absolute count of lymphocytes and monocytes per volume blood and for isolation of CD8 T cells It was also used for blood gas analysis The CD8 T cells were characterized using flow cytometry and then cultured for 7 days with CD8 activation beads The cells were counted and analyzed for viability at day4 and day8 RNAseq was performed on CD8 cells isolated before and directly after exercise CD8 T cells from Day 1 pre and post exercise and then after 7 days of culture were analyzed for IFNgamma release using Elispot and for cytotoxicity against HEK cancer cells in vitro Flow cytometry of CD8 T cells was performed on fresh CD8 T cells pre and post exercise and then on day4 and day8 after culture SeaHorse metabolic assay was done on Day1 CD8 T cells obtained pre and post exercise
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None