Ignite Creation Date:
2024-10-25 @ 7:53 PM
Last Modification Date:
2024-10-26 @ 3:35 PM
Study NCT ID:
NCT06512103
Status:
COMPLETED
Last Update Posted:
None
First Post:
2024-05-16
Brief Title:
Sclerostin Regulation in Apical Periodontitis Lesions in Apical Periodontitis Lesions
Sponsor:
None
Organization:
None
Study Overview
Official Title:
Relationship Between Bone Resorption and Sclerostin Regulation in Apical Periodontitis Lesions
Status:
COMPLETED
Status Verified Date:
2024-07
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
No
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Our aim was to examine the relationship between inflammatory bone resorption associated with the severity of AP and gingival crevicular fluid GCF and blood sclerostin and PGE2 levels in cases of AP Additionally the correlation of sclerostin regulation with RANKL and MMP-9 levels will be investigatedThis single-centered cross-sectional analytical study included 90 patients with AP and 35 systemically and orally healthy volunteers as the control group who applied for a control examination between September 2023 and February 2024 Demographic characteristics of the patients age body mass index BMI and oral hygiene index OHI and dental examination findings periapical index PAI score root canal treatment number of crowns fillings and missing teeth etc were recorded According to the PAI score the participants were divided into three groups 35 healthy patients with a PAI score of 1-2 Group 1 Control group 35 patients with a PAI score of 3-4 Group 2 mild-moderate AP and 55 patients having at least one tooth with PAI score of 5 characterized by more severe bone resorption Group 3 Severe AP Sclerostin RANKL and MMP-9 levels were measured in serum and GCF of all participants
Detailed Description:
Fasting 8-10 hours venous blood of all participants was taken from forearm antecubitalbasic veins After keeping the blood at room temperature for 30 minutes it was centrifuged at 2500 xg for 10 minutes After centrifugation the upper serum of the tubes was separated Hemolysis index HI of the sera were measured to prevent optical interference in the biochemistry autoanalyzer device Cobas 8000 Chemistry Analyzer USA Samples with a hemolysis index greater than 50 mgdl Hb were excluded from the study The sera obtained after centrifugation were aliquoted into 05 mL tubes Eppendorf Hamburg Germany and stored at - 80 C until the day of analysis
GCF samples were taken prior to periodontal probing to avoid contamination by blood To avoid contamination of the sample patients were asked not to eat or drink anything for at least 30 minutes before the procedure After selecting the area where GCF collection would be made the area of the tooth with AP and the area that often corresponds to the same area in healthy individuals the sampling area was isolated with cotton rolls and plaque was removed After gentle air-drying PerioPaper strips OraFlow Inc NY USA were placed gently until slight resistance was felt and left there for 30 seconds Three samples were taken from the mesial distal and buccal surfaces of related tooth Periopapers were thoroughly washed in 05 ml Eppendorf tubes after subtracting the tare weight of the tube with 100 µl of phosphate-buffered saline PBS using an automatic pipette Blood-stained paper strips were removed from the samples All GCF samples were weighed on a precision balance Shimadzu Libror Model AEG-220 Germany and recorded The samples in all closed tubes were mixed thoroughly with a vortex device Heidolph Reax Top Vortex Schwabach Germany for approximately 15-20 seconds This allowed GCF to pass into PBS Periopapers in tubes were removed from GCF and PBS without contamination The remaining extract in tubes was stored at -80oC until the day of analysis The results obtained on the study day were proportioned by weighing weightsPBS On the day of analysis all serums and GCF were first allowed to dissolve slowly at 4 oC and then brought to room temperature before measurement
GCF samples were taken prior to periodontal probing to avoid contamination by blood To avoid contamination of the sample patients were asked not to eat or drink anything for at least 30 minutes before the procedure After selecting the area where GCF collection would be made the area of the tooth with AP and the area that often corresponds to the same area in healthy individuals the sampling area was isolated with cotton rolls and plaque was removed After gentle air-drying PerioPaper strips OraFlow Inc NY USA were placed gently until slight resistance was felt and left there for 30 seconds Three samples were taken from the mesial distal and buccal surfaces of related tooth Periopapers were thoroughly washed in 05 ml Eppendorf tubes after subtracting the tare weight of the tube with 100 µl of phosphate-buffered saline PBS using an automatic pipette Blood-stained paper strips were removed from the samples All GCF samples were weighed on a precision balance Shimadzu Libror Model AEG-220 Germany and recorded The samples in all closed tubes were mixed thoroughly with a vortex device Heidolph Reax Top Vortex Schwabach Germany for approximately 15-20 seconds This allowed GCF to pass into PBS Periopapers in tubes were removed from GCF and PBS without contamination The remaining extract in tubes was stored at -80oC until the day of analysis The results obtained on the study day were proportioned by weighing weightsPBS On the day of analysis all serums and GCF were first allowed to dissolve slowly at 4 oC and then brought to room temperature before measurement
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None