Ignite Creation Date:
2024-10-25 @ 8:06 PM
Last Modification Date:
2024-10-26 @ 3:35 PM
Study NCT ID:
NCT06502548
Status:
NOT_YET_RECRUITING
Last Update Posted:
None
First Post:
2024-06-28
Brief Title:
Endometriosis Transcriptomic Cell Atlas
Sponsor:
None
Organization:
None
Study Overview
Official Title:
Establishing an Endometriosis Transcriptomic Cell Atlas to Decipher the Pathophysiological Role of Stem Cells and Estrogens in the Disease
Status:
NOT_YET_RECRUITING
Status Verified Date:
2024-07
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
No
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
EDISON
Brief Summary:
Endometriosis is an estrogen-dependent chronic inflammatory gynecologic disease affecting women of reproductive age with a therapeutic wandering of 6 to 10 years A better understanding of the initiation phase is a major challenge to improve diagnosis and treatment The most widely accepted hypothesis to explain the formation of endometriotic lesions is the tubal retrograde reflux during menstruation However only 10 of the reproductive age women will develop endometriosis while 90 of women experience retrograde menstruation This raises the question of the stem cells present in the endometrium and menstrual reflux of these patients but also of both the peritoneal microenvironment and the estrogenic local signaling which allow the implantation of these lesions
Detailed Description:
Using tissues collected at different phases of menstrual cycle in healthy women and patients operated for endometriotic lesions 3 main objectives
1 To characterize cellular heterogeneity between eutopic endometrium and ectopic lesions in parallel with peritoneal fluid to identify potential stem cells and the immune microenvironment in order to find new biomarkers using a combination of unbiased transcriptomic spectral flow cytometry and multiplex imaging analysis
2 To develop organoid models that integrate peritoneal fluid elements supernatant andor cells to clarify stem cell propertiescharacteristics and their supportive environment
3 To leverage the use of these endometrial derived-organoids to functionally study the differential influence of estrogen signaling in women with or without endometriosis
1 To characterize cellular heterogeneity between eutopic endometrium and ectopic lesions in parallel with peritoneal fluid to identify potential stem cells and the immune microenvironment in order to find new biomarkers using a combination of unbiased transcriptomic spectral flow cytometry and multiplex imaging analysis
2 To develop organoid models that integrate peritoneal fluid elements supernatant andor cells to clarify stem cell propertiescharacteristics and their supportive environment
3 To leverage the use of these endometrial derived-organoids to functionally study the differential influence of estrogen signaling in women with or without endometriosis
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None