Ignite Creation Date:
2024-10-26 @ 3:38 PM
Last Modification Date:
2024-10-26 @ 3:38 PM
Study NCT ID:
NCT06566495
Status:
ACTIVE_NOT_RECRUITING
Last Update Posted:
None
First Post:
2024-08-14
Brief Title:
Microorganisms on Reusable Tourniquets
Sponsor:
None
Organization:
None
Study Overview
Official Title:
Reusable Tourniquets as Potential Risk of Microbial Transmission - Comparison of Operating Theatre and Emergency Department
Status:
ACTIVE_NOT_RECRUITING
Status Verified Date:
2024-08
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
No
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
The purpose of this study was to compare microbial contamination on the surface of reusable stasis after indefinite use 2 weeks and 4 weeks We investigated how the site - the operating theater and the emergency department as well as the time of use - affects the number of organisms
Detailed Description:
The cross-sectional study was conducted in the operating theater and emergency department of a tertiary referral hospital in Gdansk Poland in three part from March to April 2024 The study included reusable tourniquets used by the hospitals medical staff during vascular access generation
After each stage of the in-hospital study the stasis was collected and replaced with new ones A total of 53 reusable stasis were collected in three phases of the study and were subjected to microbiological analysis at the Department of Immunobiology and Environmental Microbiology of the Medical University of Gdansk The tourniquets were collected into disposable sterile bags In the first stage of the study tourniquets were collected of indefinite use n 17 In the second and third stages of the study stasis used for 14 n20 and 28 n16 days respectively were collected All tourniquets were labeled and assigned to different rooms located within the surveyed wards The trial was conducted separately for the plastic fastener and the fabric band The plastic parts of the tourniquets were placed in sterile glass dishes Under laboratory conditions the plastic parts of the stasis were cut off and placed in the dishes Using sterile swabs soaked in 09 sodium chloride the plastic was swabbed after which the tip of the swab was cut off placed in a tube with 09 sodium chloride 3ml which was shaken in a Vortex device 30 seconds and the obtained material was seeded on columbia Agar with 5 sheep blood The material part of the stasis was placed in a sterile homogenization bag with the addition of 100ml of nutrient broth The bag was then placed in a Stomacher Homogenizer for two minutes to detach the microorganisms from the porous surface of the material The resulting homogenate was transferred in a concentrated state and diluted tenfold using 200 μl pipettes onto columbia Agar media supplemented with 5 sheep blood200 μl of concentrated homogenate was seeded onto the quality growth media MacConkey Broth King B Agar CHROMagar E coli and other coliforms The protected material was incubated for 24 hours at 37C in an aerobic atmosphere After this time bacterial colonies were counted and counts were performed to determine the number of microorganisms on the surface of the stasis CFUcm2 Due to the assumptions of the experiment and the time frame eight tourniquets were not included in the analysis From the information obtained from the medical staff this was due to significant soiling making it impossible to use the tourniquets and in a few cases the plastic fastener broke
After each stage of the in-hospital study the stasis was collected and replaced with new ones A total of 53 reusable stasis were collected in three phases of the study and were subjected to microbiological analysis at the Department of Immunobiology and Environmental Microbiology of the Medical University of Gdansk The tourniquets were collected into disposable sterile bags In the first stage of the study tourniquets were collected of indefinite use n 17 In the second and third stages of the study stasis used for 14 n20 and 28 n16 days respectively were collected All tourniquets were labeled and assigned to different rooms located within the surveyed wards The trial was conducted separately for the plastic fastener and the fabric band The plastic parts of the tourniquets were placed in sterile glass dishes Under laboratory conditions the plastic parts of the stasis were cut off and placed in the dishes Using sterile swabs soaked in 09 sodium chloride the plastic was swabbed after which the tip of the swab was cut off placed in a tube with 09 sodium chloride 3ml which was shaken in a Vortex device 30 seconds and the obtained material was seeded on columbia Agar with 5 sheep blood The material part of the stasis was placed in a sterile homogenization bag with the addition of 100ml of nutrient broth The bag was then placed in a Stomacher Homogenizer for two minutes to detach the microorganisms from the porous surface of the material The resulting homogenate was transferred in a concentrated state and diluted tenfold using 200 μl pipettes onto columbia Agar media supplemented with 5 sheep blood200 μl of concentrated homogenate was seeded onto the quality growth media MacConkey Broth King B Agar CHROMagar E coli and other coliforms The protected material was incubated for 24 hours at 37C in an aerobic atmosphere After this time bacterial colonies were counted and counts were performed to determine the number of microorganisms on the surface of the stasis CFUcm2 Due to the assumptions of the experiment and the time frame eight tourniquets were not included in the analysis From the information obtained from the medical staff this was due to significant soiling making it impossible to use the tourniquets and in a few cases the plastic fastener broke
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None