Ignite Creation Date:
2024-10-26 @ 3:41 PM
Last Modification Date:
2024-10-26 @ 3:41 PM
Study NCT ID:
NCT06609785
Status:
COMPLETED
Last Update Posted:
None
First Post:
2024-09-19
Brief Title:
Differential Regulation of miRNA and Protein in Human Plasma Extracellular Vesicles by Different Types of Exercise
Sponsor:
None
Organization:
None
Study Overview
Official Title:
The Relationship Between Exercise-drived Exosomes and Diabetes Mellitus
Status:
COMPLETED
Status Verified Date:
2024-09
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
No
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Aimshypothesis Both continuous aerobic training CAT and high intensity interval training HIIT are recommended to promote health and prevent diseases Exercise-induced circulating extracellular vesicles EX-EVs have been suggested to play essential roles in mediating organ crosstalk but corresponding molecular mechanisms remain largely unexplored We aimed to assess and compare the systemic effects of CAT and HIIT via multi-omics integration analysis of EX-EVs
Methods Five healthy male volunteers were assigned to HIIT 20 minutes of cycling with periods of 2 minutes at 80-95 HRmax separated by 2 minutes of active recovery and CAT 20 minutes of cycling at 60-80 of HRmax with a 7-day interval between sessions Plasma EVs were collected at rest or immediately after each training section prior to proteomics and miRNA profile analysis
Methods Five healthy male volunteers were assigned to HIIT 20 minutes of cycling with periods of 2 minutes at 80-95 HRmax separated by 2 minutes of active recovery and CAT 20 minutes of cycling at 60-80 of HRmax with a 7-day interval between sessions Plasma EVs were collected at rest or immediately after each training section prior to proteomics and miRNA profile analysis
Detailed Description:
Before the formal experiment the volunteers were undergoing a thorough physical examination and proper training to ensure that they were able to complete the experiment The participants refrained from exercise 24 hours prior to the test to ensure the integrity and accuracy of the results All volunteers had a standardized breakfast and all tests started at 900 am The volunteers successively completed both HIIT and CAT under the supervision of a professional coach and the interval between each type of exercise was 7 days Each training session was initiated with a brief 2-minute standardized warm-up followed by 20 minutes of cycling consisting of periods of 2 minutes at 80-95 maximal heart rate HRmax separated by 2 minutes of active recovery for the HIIT group or 20 minutes of cycling at 60-80 of HRmax for the CAT group A real-time heart rate monitoring system was continuously used during each training session The participants were instructed to continue their regular physical activities and eating habits throughout the intervention period Blood samples were collected at rest or immediately after each training session for further analysis
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None