Ignite Creation Date:
2024-10-26 @ 3:42 PM
Last Modification Date:
2024-10-26 @ 3:42 PM
Study NCT ID:
NCT06626711
Status:
NOT_YET_RECRUITING
Last Update Posted:
None
First Post:
2024-10-02
Brief Title:
Effect of Propofol and Sevoflurane Anesthesia on HSP70 Expression in Tumor Cells
Sponsor:
None
Organization:
None
Study Overview
Official Title:
Effect of Propofol and Sevoflurane Anesthesia on HSP70 Expression in Tumor Cells A Prospective Single-center Pilot Randomized Study
Status:
NOT_YET_RECRUITING
Status Verified Date:
2024-10
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
No
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
The goal of this clinical trial is to learn if sevoflurane-based inhalational anesthesia s-IA and propofol-based total intravenous anesthesia p-TVA have different effects on HSP70 expression in different types of tumour cells The main questions it aims to answer are
if p-TVAs-IA increase or suppress HSP70 expression in tumor cells in comparison with a pre-anesthesia level
does this change in HSP70 expression cause any difference in vital characteristics of tumor cells such as proliferation apoptosis colony formation and migration
Researchers will compare p-TVA with s-IA by ability of these types of anesthesia to change the HSP70 expression level and modulate HSP70-mediated effects in tumor cells
Participants will
be randomly allocated to p-TVA or s-IA groups
donate 12 ml of blood before anesthesia induction and 12 ml after 2 hours of anesthesia
The blood serum will be used to prepare cell medium After exposure to this medium cells from different tumor types will be investigated using cytological and molecular biological methods
if p-TVAs-IA increase or suppress HSP70 expression in tumor cells in comparison with a pre-anesthesia level
does this change in HSP70 expression cause any difference in vital characteristics of tumor cells such as proliferation apoptosis colony formation and migration
Researchers will compare p-TVA with s-IA by ability of these types of anesthesia to change the HSP70 expression level and modulate HSP70-mediated effects in tumor cells
Participants will
be randomly allocated to p-TVA or s-IA groups
donate 12 ml of blood before anesthesia induction and 12 ml after 2 hours of anesthesia
The blood serum will be used to prepare cell medium After exposure to this medium cells from different tumor types will be investigated using cytological and molecular biological methods
Detailed Description:
This study will be conducted in adult cancer patients who undergo planned radical or palliative tumor resection and signed informed consent Patients with diabetes mellitus HIV hepatitis B and C as well as patients who undergo emergency surgery will not be included to the study Patients will be randomly allocated to propofol-based TVA p-TVA and sevoflurane-based IA s-IA groups After the collection of blood before the anesthesia induction and in 2 hours of anesthesia probes will be centrifuged to prepare serum Cell medium will contain 50 of blood serum Different tumor cell lines human lung adenocarcinoma A549 large cell lung carcinoma NCI-H460 human colorectal adenocarcinoma DLD1 will be exposed to the cell medium for 4 hours what corresponds to an approximate duration of anesthesia for oncology surgery HSP70 expression level will be determined using SDS-PAGE polyacrilamide gel electrophoresis and Western-blot analysis intracellular HSP70 content ELISA of cell medium extracellular HSP70 expression and confocal microscopy
The A549 cell line with stable knockdown of HSP70 developed by shRNA will be used to distinguish HSP70-based effects of cell medium from p-TVA and s-IA groups on vital characteristics of tumor cells including proliferation MTT assay apoptosis LDH cytotoxicity assay colony formation and migration the Wound healing assay
Researchers will compare HSP70 expression levels and vital characteristics of tumor cells exposed to patient serum before and after each type of anesthesia as well as p-TVA group with s-IA group
The A549 cell line with stable knockdown of HSP70 developed by shRNA will be used to distinguish HSP70-based effects of cell medium from p-TVA and s-IA groups on vital characteristics of tumor cells including proliferation MTT assay apoptosis LDH cytotoxicity assay colony formation and migration the Wound healing assay
Researchers will compare HSP70 expression levels and vital characteristics of tumor cells exposed to patient serum before and after each type of anesthesia as well as p-TVA group with s-IA group
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None