Ignite Creation Date:
2024-10-26 @ 3:42 PM
Last Modification Date:
2024-10-26 @ 3:42 PM
Study NCT ID:
NCT06631820
Status:
NOT_YET_RECRUITING
Last Update Posted:
None
First Post:
2024-10-03
Brief Title:
The Effect of the Nutraceutical -- Epicatechin on Myosteatosis in Patients with Advanced CKD
Sponsor:
None
Organization:
None
Study Overview
Official Title:
The Effect of the Nutraceutical -- Epicatechin on Myosteatosis in Patients with Advanced CKD a Pilot Study EPIC-CKD
Status:
NOT_YET_RECRUITING
Status Verified Date:
2024-10
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
No
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
EPIC-CKD
Brief Summary:
Some foods have components that can prevent and treat some diseases and provide beneficial effects for health These components naturally occurring in foods are called nutraceuticals Recently it was found that Epicatechin which is a kind of nutraceutical from the Catechins family naturally occurring in green tea and cocoa has positive effects on muscle mass and strength of people with chronic diseases
People with chronic kidney disease often present muscle loss and lack of strength that are not easily treated with regular diet and physical activity We here propose a study where 10 individuals with chronic kidney disease will receive a dose of Epicatechin for 8 weeks and we aim to test if Epicatechin improves general muscle health Based on what is known we expect to see an increase in muscle mass and strength
The dose of Epicatechin provided 100 mgday is safe since it is much lower than the dose usually available in supplements In addition this is the form naturally present in green tea and cocoa and in similar amounts Subjects participating in the study will be evaluated for muscle mass and strength three times during the study Muscle mass and muscle quality will be evaluated by ultrasound and magnetic resonance imaging The participants will also perform some tests like walking in a corridor and seating and standing from a chair to measure their strength and performance Adverse side effects will be monitored via telephone and safety will be assessed by monthly blood tests that will evaluate liver and kidney function If this study shows that Epicatechin can promote muscle growth and strength it will positively affect patients with chronic kidney disease that might benefit of a natural substance from food to improve muscle health
People with chronic kidney disease often present muscle loss and lack of strength that are not easily treated with regular diet and physical activity We here propose a study where 10 individuals with chronic kidney disease will receive a dose of Epicatechin for 8 weeks and we aim to test if Epicatechin improves general muscle health Based on what is known we expect to see an increase in muscle mass and strength
The dose of Epicatechin provided 100 mgday is safe since it is much lower than the dose usually available in supplements In addition this is the form naturally present in green tea and cocoa and in similar amounts Subjects participating in the study will be evaluated for muscle mass and strength three times during the study Muscle mass and muscle quality will be evaluated by ultrasound and magnetic resonance imaging The participants will also perform some tests like walking in a corridor and seating and standing from a chair to measure their strength and performance Adverse side effects will be monitored via telephone and safety will be assessed by monthly blood tests that will evaluate liver and kidney function If this study shows that Epicatechin can promote muscle growth and strength it will positively affect patients with chronic kidney disease that might benefit of a natural substance from food to improve muscle health
Detailed Description:
The effect of ageing and chronic diseases on the skeletal muscle is of great interest for studies in clinical nutrition In the setting of chronic kidney disease CKD CKD-related metabolic alterations act synergistically with other risk factors in the deterioration of muscle health Loss of muscle mass and the replacement of contractile muscle fibers by adipose tissue ie myosteatosis have been shown to decrease muscle strength and function and have a negative effect on patient centred outcomes such as quality of life
Considering that patients with low strength have an increased risk of falling less independency to perform routine tasks and are more vulnerable to stressors with increased risk for recurring hospitalizations infections and mortality it is of utmost importance to explore treatment strategies to overcome muscle abnormalities while improving function Recently interventional trials using the nutraceutical -- Epicatechin an abundant flavanol present in cacao and green tea showed an increase in skeletal muscle mass and exercise capacity through stimulating mitochondrial biogenesis On this regard this pilot study aims to evaluate if 100mg of -- Epicatechin can help ameliorate muscle abnormalities in patients with CKD
Aims This study aims
1 To evaluate the effect of nutraceutical -- Epicatechin on myosteatosis and functional capacity of patients with advanced CKD
2 To evaluate the effect of nutraceutical -- Epicatechin on markers of muscle regeneration
3 To evaluate the effect of nutraceutical -- Epicatechin on markers of mitochondrial dysfunction
This project is a feasibility pilot study to test the effect of the nutraceutical -- Epicatechin on muscle health To allow for a control phase this study has a cross-over with open label design We here describe the methodological approach that will allow us to achieve the proposed aims
Patients will be pre-selected from medical records and contacted telephonically by the investigators to be invited to participate if they accept they will be invited to come to the research center to receive an explanation regarding the study sign the informed consent and undergo a screening visit
Sample size estimation In this pilot study we will utilize a convenience sample of 10 subjects
Study design and intervention After enrolment patients will be followed for 8 weeks without receiving the nutraceutical After this control period patients will take 50 mg of -- Epicatechin twice a day for 8 weeks total dose 100mgday The nutraceutical will be supplied as monodose sachets from Aayam TherapeuticsStudy participants will mix the sachet powder with juice 10-20mL in the morning 15 minutes before breakfast and in the evening 15 minutes before sleeping or 1h after dinner
The study will comprise 5 visits in total The first visit will be a screening visit During the screening visit patients will be oriented to not change their diets during the study period and they will be instructed on how to collect 24h urine to bring at the baseline visit After 1-2 weeks patients will come back for the baseline visit visit 1 when they will undergo all evaluations and sampling as described belowThe interval between first and second visit is of 8 weeks At week 8 visit 2 patients will undergo all study evaluations and sampling will bring another 24h urine collection and will receive the supplements to be used in the next 8 weeks After 4 weeks receiving the supplement patients will receive a phone call visit 3 to enquire about gastrointestinal symptoms and to be invited to go to their local vårdcentral to perform the safety blood tests see below After another 4 weeks patients will come back for the end-of -study visit visit 4 in which they will perform all study evaluations and sampling again
The evaluation at patients local vårdcentral can take place any day during the selected week while the end-of-study visit must take place within 1 week of the end of week 16 see detailed picture below
The duration of the 3 main visits is expected to be 3-4h patients will receive food after blood collection in a fasting state
Study variables
Clinical and demographic variables age sex Charlson comorbidity index aetiology of the nephropathy will be recorded at baseline visit
Nutritional and body composition assessment nutritional evaluation will be performed at baseline week 8 and week 16
Anthropometric variables Body weight height waist circumference calf circumference
Body composition variables
Total body skeletal muscle and phase angle will be measured with bioimpedance analysis
Myosteatosis and skeletal muscle quantity of patients will be assessed with 2 imaging modalities Magnetic Resonance Imaging MRI and Ultrasound US MRI and US will be used to assess the level of myosteatosis and markers of muscle mass of the quadriceps muscle USs reliability and validity in assessing muscle quantity and myosteatosis will be studied in comparison to MRI
Measurements will be performed at the mid-point between the upper pole of the patella and the anterior superior iliac spine in the dominant leg US will be performed before the MRI and a vitamin D pill will be used to marc the spot for the MRI assessment
US We will use a B-mode wall tracking US device Clarius Vancouver Canada already available at the research center of Karolinska Institute Measurements will be performed by a researcher with extensive experience in assessing body composition by using US and will follow a standardized protocol applied repeatedly in patients with acute and chronic kidney disease Measurements dont take more than 10 minutes All images will be stored anonymously and measurements will be performed afterwards by using the software ImageJ httpsimagejnihgovijindexhtml Two muscles will be identified the rectus femoris and vastus intermedius The collected images will allow us to measure muscle thickness rectus femoris cross-sectional area rectus femoris and vastus intermedius echogenicity While thickness and cross-sectional area provide information on muscle quantity the echogenicity of the muscle provides information on myosteatosis
MRI We will evaluate rectus femoris volume rectus femoris and vastus intermedius thickness and rectus femoris cross-sectional area to evaluate muscle quantity Myosteatosis will be evaluated by applying quantitative fat water imaging Dixon imaging information on intermuscular adipose tissue IMAT intramuscular adipose tissue and average myosteatosis level will be performed by experienced radiologist
Functional tests Strength will be assessed by handgrip strength using a portable dynamometer and by the chair stand test 5 time sit to stand and 30 seconds sit-to stand test Performance will be assessed by the gait speed test that evaluates the normal speed that a person usually walks and by the 6 minutes walking distance test An integrated functional test called Short Physical Performance Battery SPPB will be used at baseline week 8 and week
Safety assessment Monitoring will occur at baseline week 8 in person visit week 12 by phone call and local vårdcentral for exams and week 16 in person visit Safety will be assessed by frequent monitoring of adverse events gastrointestinal symptoms and blood parameters ie aspartate aminotransferase ASAT alanine aminotransferase ALAT and serum creatinine
Laboratorial data For the screening visit after signing the informed consent patients will undergo blood sampling for assessment of serum creatinine and liver function
At study baseline week 8 and week 16 patients will perform the following blood tests in a 8h fasting state serum creatinine ASATALAT glycemia HbA1c total cholesterol uric acid PTH free testosterone LDL cholesterol HDL cholesterol Triglycerides and CRP
Blood samples will be stored for posterior evaluation of oxidative stress markers protein carbonylation 8-hydroxy-2-deoxyguanosine 8-OhdG epigenetic clock DNA metilation clocks evaluation microbial DNA as an exploratory analysis to assess the effect of epicatechin on gut microbiota and specific markers that will allow us to achieve aims 2 and 3 see below
Markers to evaluate aim 2 blood markers that signals for muscle regeneration are myostatin follistatin and GDF-11
Markers to evaluate aim 3 blood markers that evaluate mitochondrial dysfunction include lactate pyruvate mitochondrial copy numbers creatine phosphokinase FGF-21 GDF-15
Patients will also bring a 24h urine sample from the day before the visit to measure urinary urea nitrogen sodiuria calciuria and oxaluria The urine sample will not be stored
Data on patients medication will be collected at every main visit with special attention to erythropoietin usage Patients will also receive an activity monitor at baseline to be used during the entire study period
Adherence will be evaluated based on the supplements leftovers at the end of the study
Significance
To treat sarcopenia and myosteatosis in CKD is a challenge Patients with advanced CKD have anabolic resistance which is defined by a reduced stimulation in muscle protein synthesis after anabolic stimuli such as exercise and dietary protein and is also a feature of mitochondrial dysfunction This project innovates by testing a nutraceutical available in various food sources but especially in cacao and green tea that could help overcome this condition of anabolic resistance If our hypotheses are confirmed the nutraceutical -- Epicatechin could be used as an adjuvant factor in the treatment of sarcopenia and myosteatosis in patients with advanced CKD
Considering that patients with low strength have an increased risk of falling less independency to perform routine tasks and are more vulnerable to stressors with increased risk for recurring hospitalizations infections and mortality it is of utmost importance to explore treatment strategies to overcome muscle abnormalities while improving function Recently interventional trials using the nutraceutical -- Epicatechin an abundant flavanol present in cacao and green tea showed an increase in skeletal muscle mass and exercise capacity through stimulating mitochondrial biogenesis On this regard this pilot study aims to evaluate if 100mg of -- Epicatechin can help ameliorate muscle abnormalities in patients with CKD
Aims This study aims
1 To evaluate the effect of nutraceutical -- Epicatechin on myosteatosis and functional capacity of patients with advanced CKD
2 To evaluate the effect of nutraceutical -- Epicatechin on markers of muscle regeneration
3 To evaluate the effect of nutraceutical -- Epicatechin on markers of mitochondrial dysfunction
This project is a feasibility pilot study to test the effect of the nutraceutical -- Epicatechin on muscle health To allow for a control phase this study has a cross-over with open label design We here describe the methodological approach that will allow us to achieve the proposed aims
Patients will be pre-selected from medical records and contacted telephonically by the investigators to be invited to participate if they accept they will be invited to come to the research center to receive an explanation regarding the study sign the informed consent and undergo a screening visit
Sample size estimation In this pilot study we will utilize a convenience sample of 10 subjects
Study design and intervention After enrolment patients will be followed for 8 weeks without receiving the nutraceutical After this control period patients will take 50 mg of -- Epicatechin twice a day for 8 weeks total dose 100mgday The nutraceutical will be supplied as monodose sachets from Aayam TherapeuticsStudy participants will mix the sachet powder with juice 10-20mL in the morning 15 minutes before breakfast and in the evening 15 minutes before sleeping or 1h after dinner
The study will comprise 5 visits in total The first visit will be a screening visit During the screening visit patients will be oriented to not change their diets during the study period and they will be instructed on how to collect 24h urine to bring at the baseline visit After 1-2 weeks patients will come back for the baseline visit visit 1 when they will undergo all evaluations and sampling as described belowThe interval between first and second visit is of 8 weeks At week 8 visit 2 patients will undergo all study evaluations and sampling will bring another 24h urine collection and will receive the supplements to be used in the next 8 weeks After 4 weeks receiving the supplement patients will receive a phone call visit 3 to enquire about gastrointestinal symptoms and to be invited to go to their local vårdcentral to perform the safety blood tests see below After another 4 weeks patients will come back for the end-of -study visit visit 4 in which they will perform all study evaluations and sampling again
The evaluation at patients local vårdcentral can take place any day during the selected week while the end-of-study visit must take place within 1 week of the end of week 16 see detailed picture below
The duration of the 3 main visits is expected to be 3-4h patients will receive food after blood collection in a fasting state
Study variables
Clinical and demographic variables age sex Charlson comorbidity index aetiology of the nephropathy will be recorded at baseline visit
Nutritional and body composition assessment nutritional evaluation will be performed at baseline week 8 and week 16
Anthropometric variables Body weight height waist circumference calf circumference
Body composition variables
Total body skeletal muscle and phase angle will be measured with bioimpedance analysis
Myosteatosis and skeletal muscle quantity of patients will be assessed with 2 imaging modalities Magnetic Resonance Imaging MRI and Ultrasound US MRI and US will be used to assess the level of myosteatosis and markers of muscle mass of the quadriceps muscle USs reliability and validity in assessing muscle quantity and myosteatosis will be studied in comparison to MRI
Measurements will be performed at the mid-point between the upper pole of the patella and the anterior superior iliac spine in the dominant leg US will be performed before the MRI and a vitamin D pill will be used to marc the spot for the MRI assessment
US We will use a B-mode wall tracking US device Clarius Vancouver Canada already available at the research center of Karolinska Institute Measurements will be performed by a researcher with extensive experience in assessing body composition by using US and will follow a standardized protocol applied repeatedly in patients with acute and chronic kidney disease Measurements dont take more than 10 minutes All images will be stored anonymously and measurements will be performed afterwards by using the software ImageJ httpsimagejnihgovijindexhtml Two muscles will be identified the rectus femoris and vastus intermedius The collected images will allow us to measure muscle thickness rectus femoris cross-sectional area rectus femoris and vastus intermedius echogenicity While thickness and cross-sectional area provide information on muscle quantity the echogenicity of the muscle provides information on myosteatosis
MRI We will evaluate rectus femoris volume rectus femoris and vastus intermedius thickness and rectus femoris cross-sectional area to evaluate muscle quantity Myosteatosis will be evaluated by applying quantitative fat water imaging Dixon imaging information on intermuscular adipose tissue IMAT intramuscular adipose tissue and average myosteatosis level will be performed by experienced radiologist
Functional tests Strength will be assessed by handgrip strength using a portable dynamometer and by the chair stand test 5 time sit to stand and 30 seconds sit-to stand test Performance will be assessed by the gait speed test that evaluates the normal speed that a person usually walks and by the 6 minutes walking distance test An integrated functional test called Short Physical Performance Battery SPPB will be used at baseline week 8 and week
Safety assessment Monitoring will occur at baseline week 8 in person visit week 12 by phone call and local vårdcentral for exams and week 16 in person visit Safety will be assessed by frequent monitoring of adverse events gastrointestinal symptoms and blood parameters ie aspartate aminotransferase ASAT alanine aminotransferase ALAT and serum creatinine
Laboratorial data For the screening visit after signing the informed consent patients will undergo blood sampling for assessment of serum creatinine and liver function
At study baseline week 8 and week 16 patients will perform the following blood tests in a 8h fasting state serum creatinine ASATALAT glycemia HbA1c total cholesterol uric acid PTH free testosterone LDL cholesterol HDL cholesterol Triglycerides and CRP
Blood samples will be stored for posterior evaluation of oxidative stress markers protein carbonylation 8-hydroxy-2-deoxyguanosine 8-OhdG epigenetic clock DNA metilation clocks evaluation microbial DNA as an exploratory analysis to assess the effect of epicatechin on gut microbiota and specific markers that will allow us to achieve aims 2 and 3 see below
Markers to evaluate aim 2 blood markers that signals for muscle regeneration are myostatin follistatin and GDF-11
Markers to evaluate aim 3 blood markers that evaluate mitochondrial dysfunction include lactate pyruvate mitochondrial copy numbers creatine phosphokinase FGF-21 GDF-15
Patients will also bring a 24h urine sample from the day before the visit to measure urinary urea nitrogen sodiuria calciuria and oxaluria The urine sample will not be stored
Data on patients medication will be collected at every main visit with special attention to erythropoietin usage Patients will also receive an activity monitor at baseline to be used during the entire study period
Adherence will be evaluated based on the supplements leftovers at the end of the study
Significance
To treat sarcopenia and myosteatosis in CKD is a challenge Patients with advanced CKD have anabolic resistance which is defined by a reduced stimulation in muscle protein synthesis after anabolic stimuli such as exercise and dietary protein and is also a feature of mitochondrial dysfunction This project innovates by testing a nutraceutical available in various food sources but especially in cacao and green tea that could help overcome this condition of anabolic resistance If our hypotheses are confirmed the nutraceutical -- Epicatechin could be used as an adjuvant factor in the treatment of sarcopenia and myosteatosis in patients with advanced CKD
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None