Ignite Creation Date:
2024-05-05 @ 11:22 AM
Last Modification Date:
2024-10-26 @ 9:05 AM
Study NCT ID:
NCT00006314
Status:
COMPLETED
Last Update Posted:
2016-05-13
First Post:
2000-09-28
Brief Title:
Cytomegalovirus Spread and Reactivation in Blood Cells
Sponsor:
National Heart Lung and Blood Institute NHLBI
Organization:
National Heart Lung and Blood Institute NHLBI
Study Overview
Official Title:
None
Status:
COMPLETED
Status Verified Date:
2006-01
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
To investigate the relationship between HCMV and bone marrow progenitor cells to understand whether HCMV is latent in CD34 bone marrow progenitors and the mechanism by which the virus remains in a latent state
Detailed Description:
BACKGROUND
Despite progress in understanding the pathophysiology of human cytomegalovirus HCMV infections its manifestations in the immune compromised host are frequently associated with high morbidity and mortality In this setting HCMV disease can develop eg following immune suppression as a result of reactivation of latent HCMV acquired earlier in life The mechanisms leading to establishment of latent infections and their subsequent reactivation are not clear It is also unknown whether HCMV exists in a latent form with limited viral gene expression or as a persistent infection with normal virus transcription
DESIGN NARRATIVE
The specific aims of the study were to 1 examine the percentage of HCMV positive donors whose bone marrow progenitors contained HCMV DNA using nested PCR and determine if virus could be rescued from those cells 2 Analyze the HCMV life cycle in hematopoietic progenitor and stem cells 3 identify and analyze HCMV gene expression in in vivo infected leukocytes Bone marrow progenitors containing HCMV DNA detectable by nested PCR were isolated from human donors and used as as source of mRNA to prepare Cdna libraries 4 Determine if genes expressed in bone marrow progenitors were important in either establishing or maintaining a latent infection or in the lytic cycle of HCMV Information provided from the above studies yielded information important in planning future approaches for the therapy of HCMV infections
The study completion date listed in this record was obtained from the End Date entered in the Protocol Registration and Results System PRS record
Despite progress in understanding the pathophysiology of human cytomegalovirus HCMV infections its manifestations in the immune compromised host are frequently associated with high morbidity and mortality In this setting HCMV disease can develop eg following immune suppression as a result of reactivation of latent HCMV acquired earlier in life The mechanisms leading to establishment of latent infections and their subsequent reactivation are not clear It is also unknown whether HCMV exists in a latent form with limited viral gene expression or as a persistent infection with normal virus transcription
DESIGN NARRATIVE
The specific aims of the study were to 1 examine the percentage of HCMV positive donors whose bone marrow progenitors contained HCMV DNA using nested PCR and determine if virus could be rescued from those cells 2 Analyze the HCMV life cycle in hematopoietic progenitor and stem cells 3 identify and analyze HCMV gene expression in in vivo infected leukocytes Bone marrow progenitors containing HCMV DNA detectable by nested PCR were isolated from human donors and used as as source of mRNA to prepare Cdna libraries 4 Determine if genes expressed in bone marrow progenitors were important in either establishing or maintaining a latent infection or in the lytic cycle of HCMV Information provided from the above studies yielded information important in planning future approaches for the therapy of HCMV infections
The study completion date listed in this record was obtained from the End Date entered in the Protocol Registration and Results System PRS record
Study Oversight
Has Oversight DMC:
Is a FDA Regulated Drug?:
Is a FDA Regulated Device?:
Is an Unapproved Device?:
Is a PPSD?:
Is a US Export?:
Is an FDA AA801 Violation?:
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| R01HL063470 | NIH | None | httpsreporternihgovquickSearchR01HL063470 |