Ignite Creation Date:
2024-05-05 @ 11:25 AM
Last Modification Date:
2024-10-26 @ 9:07 AM
Study NCT ID:
NCT00035698
Status:
COMPLETED
Last Update Posted:
2017-03-17
First Post:
2002-05-04
Brief Title:
Epidemiological and Genetic Studies of Body Mass Index
Sponsor:
Boston University
Organization:
Boston University
Study Overview
Official Title:
Epidemiological and Genetic Studies of Body Mass Index
Status:
COMPLETED
Status Verified Date:
2017-03
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
To identify genes involved in obesity
Detailed Description:
BACKGROUND
Increased levels of body mass index BMI are associated with increased mortality and morbidity from cardiovascular disease hypertension diabetes and other disorders The frequency of obesity and its associated health-related problems is increasing in the American population
DESIGN NARRATIVE
The study builds upon a two-stage genome scan for BMI performed in the NHLBI Family Heart Study FHS In the first 101 pedigrees were examined with 1027 persons genotyped and a LOD of 22 was found on chromosome 7 In stage 2 135 sibships of 380 persons were examined and a LOD of 32 was found for the same locus Compelling linkage was found in the combined study LOD 49 chr 7q313 137cM The LOD or logarithm of odds is a statistical estimate of whether two loci the sites of genes are likely to lie near each other on a chromosome and are therefore likely to be inherited together as a package
A novel strategy will be used which combines three cutting edge methods 1 Regression Tree analyses to identify a homogenous subset of families with evidence for BMI linkage to 7q313 2 DNA pooling of samples from linked versus unlinked families and 3 quantitative PCR of DNA pools for very high-density single nucleotide polymorphism SNP mapping The combination of these methods will permit a cost effective approach for the identification of genetic polymorphisms in linkage disequilibrium with BMI and has the potential to become a widely adopted method for gene localization of complex traits
The study was extended through January 2008 to show compelling evidence for a haplotype in the 5 region of the Leptin gene p000005 influencing BMI among men in the sample The study will further demonstrate that the responsible gene in this region is not Leptin SNP and haplotype association studies implicate three strong candidate loci and other loci also warrant additional study The study will confirm SNP association in an independent study of 200 families showing linkage to the same position from Dr R Arlen Prices group Those loci with confirmed association will be further characterized by sequencing genotyping new polymorphisms and gene expression studies to identify the responsible genes
Increased levels of body mass index BMI are associated with increased mortality and morbidity from cardiovascular disease hypertension diabetes and other disorders The frequency of obesity and its associated health-related problems is increasing in the American population
DESIGN NARRATIVE
The study builds upon a two-stage genome scan for BMI performed in the NHLBI Family Heart Study FHS In the first 101 pedigrees were examined with 1027 persons genotyped and a LOD of 22 was found on chromosome 7 In stage 2 135 sibships of 380 persons were examined and a LOD of 32 was found for the same locus Compelling linkage was found in the combined study LOD 49 chr 7q313 137cM The LOD or logarithm of odds is a statistical estimate of whether two loci the sites of genes are likely to lie near each other on a chromosome and are therefore likely to be inherited together as a package
A novel strategy will be used which combines three cutting edge methods 1 Regression Tree analyses to identify a homogenous subset of families with evidence for BMI linkage to 7q313 2 DNA pooling of samples from linked versus unlinked families and 3 quantitative PCR of DNA pools for very high-density single nucleotide polymorphism SNP mapping The combination of these methods will permit a cost effective approach for the identification of genetic polymorphisms in linkage disequilibrium with BMI and has the potential to become a widely adopted method for gene localization of complex traits
The study was extended through January 2008 to show compelling evidence for a haplotype in the 5 region of the Leptin gene p000005 influencing BMI among men in the sample The study will further demonstrate that the responsible gene in this region is not Leptin SNP and haplotype association studies implicate three strong candidate loci and other loci also warrant additional study The study will confirm SNP association in an independent study of 200 families showing linkage to the same position from Dr R Arlen Prices group Those loci with confirmed association will be further characterized by sequencing genotyping new polymorphisms and gene expression studies to identify the responsible genes
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| R01HL068891 | NIH | None | httpsreporternihgovquickSearchR01HL068891 |