Ignite Creation Date:
2024-05-05 @ 10:00 AM
Last Modification Date:
2024-10-26 @ 9:05 AM
Study NCT ID:
NCT00005465
Status:
COMPLETED
Last Update Posted:
2016-02-10
First Post:
2000-05-25
Brief Title:
Genetic Mapping of Atherogenic Lipoprotein Phenotypes
Sponsor:
University of Washington
Organization:
University of Washington
Study Overview
Official Title:
None
Status:
COMPLETED
Status Verified Date:
2001-05
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
To map the major gene influencing low-density lipoprotein subclass phenotypes denoted atherogenic lipoprotein ALP phenotypes with a long term goal of cloning the ALP gene and understanding its role in genetic susceptibility to atherosclerosis
Detailed Description:
BACKGROUND
ALP phenotype B ALP-B characterized by a predominance of small dense LDL particles as determined by gradient gel electrophoresis has been associated with increased risk of myocardial infarction and a constellation of atherogenic lipid and apolipoprotein apo changes Based on complex segregation analysis ALP-B appeared to be influenced by a single major genetic locus with a dominant mode of inheritance and a common allele frequency This project was designed to identify a new gene involved in susceptibility to coronary heart disease
DESIGN NARRATIVE
The investigators identified collected and constructed a repository of immortalized cell lines and lipid and apo measurements from members of families informative for ALP They tested genes implicated in lipoprotein metabolism as possible candidate ALP genes and used highly informative DNA probes to search the genome for linkage to the ALP gene They also refined the model for the inheritance of ALP phenotypes and tested for genetic-environmental interactions Forty informative families were recruited for the repository The families were identified through two sources of probands former participants in a cholesterol-lowering diet study and patients seen at the lipid clinics at the University of Washington Each participating family member completed a medical history questionnaire and provided a blood sample for ALP phenotype determination for DNA studies and for lipid and apo measurements Linkage studies and LOD score analyses began with a candidate gene approach and continued by using DNA probes that revealed restriction fragment length polymorphisms RFLPs to search the genome for linkage to the ALP gene When a linkage was found ALP genotype information was used to refine the statistical model describing the inheritance of ALP phenotypes and to evaluate genetic-environmental interactions involving lipid and apo levels and environmental and behavioral factors
ALP phenotype B ALP-B characterized by a predominance of small dense LDL particles as determined by gradient gel electrophoresis has been associated with increased risk of myocardial infarction and a constellation of atherogenic lipid and apolipoprotein apo changes Based on complex segregation analysis ALP-B appeared to be influenced by a single major genetic locus with a dominant mode of inheritance and a common allele frequency This project was designed to identify a new gene involved in susceptibility to coronary heart disease
DESIGN NARRATIVE
The investigators identified collected and constructed a repository of immortalized cell lines and lipid and apo measurements from members of families informative for ALP They tested genes implicated in lipoprotein metabolism as possible candidate ALP genes and used highly informative DNA probes to search the genome for linkage to the ALP gene They also refined the model for the inheritance of ALP phenotypes and tested for genetic-environmental interactions Forty informative families were recruited for the repository The families were identified through two sources of probands former participants in a cholesterol-lowering diet study and patients seen at the lipid clinics at the University of Washington Each participating family member completed a medical history questionnaire and provided a blood sample for ALP phenotype determination for DNA studies and for lipid and apo measurements Linkage studies and LOD score analyses began with a candidate gene approach and continued by using DNA probes that revealed restriction fragment length polymorphisms RFLPs to search the genome for linkage to the ALP gene When a linkage was found ALP genotype information was used to refine the statistical model describing the inheritance of ALP phenotypes and to evaluate genetic-environmental interactions involving lipid and apo levels and environmental and behavioral factors
Study Oversight
Has Oversight DMC:
Is a FDA Regulated Drug?:
Is a FDA Regulated Device?:
Is an Unapproved Device?:
Is a PPSD?:
Is a US Export?:
Is an FDA AA801 Violation?:
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| R01HL046880 | NIH | None | httpsreporternihgovquickSearchR01HL046880 |