Ignite Creation Date:
2024-05-05 @ 11:05 PM
Last Modification Date:
2024-10-26 @ 10:28 AM
Study NCT ID:
NCT01250080
Status:
COMPLETED
Last Update Posted:
2010-11-30
First Post:
2010-11-29
Brief Title:
Effect of Gutamine Administration in the Innate Immune System Response in ICU Patients
Sponsor:
Hospital Universitari Son Dureta
Organization:
Hospital Universitari Son Dureta
Study Overview
Official Title:
None
Status:
COMPLETED
Status Verified Date:
2008-09
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Glutamine is the most abundant nonessential amino acid in the human body Besides its role as a constituent of proteins and its importance in amino acid transamination glutamine may modulate immune cells
The innate immune system is the first line of host defence against pathogens and in most cases sufficient to eliminate invading microbes Mammalian Toll-like receptors TLR comprise a family of germ line-encoded trans-membrane receptors which activation leads to the induction of inflammatory responses phagocytosis but also to the development of antigen specific adapative immunity
It has been postulated though not formally proven yet that glutamine beneficial effect could be due to a positive effect on the innate immune system Given the importance of TLRs and TLRs-dependent signalling in host defence against infections we hypothesized that glutamine may increase the expression andor functionality of TLRs which in turn may have beneficial effects to clear infections
The innate immune system is the first line of host defence against pathogens and in most cases sufficient to eliminate invading microbes Mammalian Toll-like receptors TLR comprise a family of germ line-encoded trans-membrane receptors which activation leads to the induction of inflammatory responses phagocytosis but also to the development of antigen specific adapative immunity
It has been postulated though not formally proven yet that glutamine beneficial effect could be due to a positive effect on the innate immune system Given the importance of TLRs and TLRs-dependent signalling in host defence against infections we hypothesized that glutamine may increase the expression andor functionality of TLRs which in turn may have beneficial effects to clear infections
Detailed Description:
Objective To evaluate whether glutamine supplementation alters the expression and functionality of TLR2 and TLR4 in circulating monocytes of trauma patients admitted to the ICU Specifically the next variables were measured
Expression of TLR2 and TLR4 in peripheral blood monocytes was determined by flow cytometry
To study the functionality of TLR2 and TLR4 monocytes were stimulated with TLR specific agonists and cytokines were measured in cell culture supernatants We determined the concentration of IL-1β IL-6 TNFα and IL-10 in cell culture supernatants using a bead array ELISA
To determine the phagocytic capability of monocytes live Escherichia coli expressing green fluorescent protein was added to 100 μL of whole blood collected in K2-anticoagulation medium tubes Bacteria were added at a ratio of 100 bacteria per monocyte The analyses were carried out in an Epics XL flow cytometer
Expression of TLR2 and TLR4 in peripheral blood monocytes was determined by flow cytometry
To study the functionality of TLR2 and TLR4 monocytes were stimulated with TLR specific agonists and cytokines were measured in cell culture supernatants We determined the concentration of IL-1β IL-6 TNFα and IL-10 in cell culture supernatants using a bead array ELISA
To determine the phagocytic capability of monocytes live Escherichia coli expressing green fluorescent protein was added to 100 μL of whole blood collected in K2-anticoagulation medium tubes Bacteria were added at a ratio of 100 bacteria per monocyte The analyses were carried out in an Epics XL flow cytometer
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None