Ignite Creation Date:
2024-06-16 @ 11:48 AM
Last Modification Date:
2024-10-26 @ 3:30 PM
Study NCT ID:
NCT06426758
Status:
NOT_YET_RECRUITING
Last Update Posted:
2024-05-23
First Post:
2024-05-19
Brief Title:
Graves Disease Induced by Epstein-Barr Virus Lytic Reactivation
Sponsor:
Organization:
Nanjing Medical University
Study Design
Study Type:
OBSERVATIONAL
Expanded Access Type Individual:
None
Expanded Access Type Intermediate:
None
Expanded Access Type Treatment:
None
Patient Registry:
False
Target Duration:
None
Design Allocation:
Design Intervention Model:
Design Intervention Model Description:
Design Masking Description:
Bio Spec Retention:
SAMPLES_WITH_DNA
Bio Spec Description:
Following DNA isolation with a QIAamp DNA Blood Mini Kit the number of EBV copies in PBMCs was detected using real-time PCR assay by means of the ISEX variant of the EBV PCR kit The results are expressed in units of EBV DNA copiesµg of DNA EBV DNA copiesmL of PBMCs suspension and EBV DNAcopies100000 cells
Peripheral blood samples were collected from patients with GD and recurrence of hyperthyroidismTotal RNA was extracted from blood using the conventional TRIzol method Reverse transcription was performed using PrimeScript RT Reagent Kit with cDNA Eraser for two-step reverse transcription polymerase chain reaction
Enrollment Count:
300
Enrollment Type:
ESTIMATED
Design Primary Purpose:
Design Masking:
Phases:
Observational Models:
| Name |
|---|
| Cohort |
Time Perspective List:
Clinical Trial Statuses
| Name |
|---|
| 107165 |
Who Masked List: