Assay Name:
TOX21_AR_LUC_MDAKB2_Agonist_3uM_Nilutamide
Organism:
human
Footprint:
microplate: 1536-well plate
Format:
cell-based
Cell Format:
cell line
Description:
TOX21_AR_LUC_MDAKB2_Agonist_3uM_Nilutamide is a cell-based, single-readout assay that uses MDA-kb2, a human breast cell line, with measurements taken at 24 hours after chemical dosing in a 1536-well plate. This is an secondary assay to TOX21_AR_LUC_MDAKB2_Agonist for agonist specificity. This particular screening for measuring luciferase reporter gene activity using MDA-kb2 cells against Tox21 10K library compounds is a confirmation study on agonist mode screening in the presence of AR-antagonist compound (Nilutamide) at 3.0uM final concentration.
Time in Hours:
24.00
Assay Format Type Sub:
cell-based format
Cell Free Component Source:
NA
Cell Growth Mode:
adherent