Assay Name:
ATG_XSP2_multi-species_TRANS
Organism:
human
Footprint:
microplate: 24-well plate
Format:
cell-based
Cell Format:
cell line
Description:
ATG_XSP2_multi-species_TRANS is a cell-based, multiplexed assay created by modifying the existing Attagene TRANS-FACTORIAL system to include a panel of nuclear receptors from some or all the following species: human (Homo sapiens), mouse (Mus musculus), frog (Xenopus laevis), zebrafish (Danio rerio), chicken (Gallus gallus), and turtle (Chrysemys picta). The ECOTOX-FACTORIAL format uses HepG2, a human liver cell line, with measurements taken at 24 hours after chemical dosing in a 24-well plate. Xsp2 included 3 nM 6-alpha-fluorotestosterone and 1.5 nM norgestrel to stimulate the androgen receptor.
Time in Hours:
24.00
Assay Format Type Sub:
cell-based format
Cell Free Component Source:
NA
Cell Growth Mode:
adherent