Assay Name:
CPHEA_Stoker_NIS
Organism:
human
Footprint:
microplate: 96-well plate
Format:
cell-based
Cell Format:
cell line
Description:
CPHEA_Stoker_NIS is a cell-based, single-readout assay that uses hNIS-HEK293T-EPA, a novel human kidney cell line expressing human NIS, with measurements taken at 2 hours after chemical dosing in a 96-well plate. This radioactive iodide uptake (RAIU) assay is used to identify inhibitors of NIS-mediated iodide uptake. A parallel cytoxicity assay (Cell Titer Glo, Promega) was also run in the same cell line.
Time in Hours:
2.00
Assay Format Type Sub:
cell-based format
Cell Free Component Source:
Cell Growth Mode:
adherent