Assay Name:
TOX21_PR_BLA_Agonist
Organism:
human
Footprint:
microplate: 1536-well plate
Format:
cell-based
Cell Format:
cell line
Description:
TOX21_PR_BLA_Agonist is a cell-based, single-readout assay that uses transfected HEK293T, a human kidney cell line, with measurements taken at 24 hours after chemical dosing in a 1536-well plate. To identify the compounds that stimulate PR signaling, a PR-UAS-bla GripTite cell line i.e. PR-UAS-bla HEK293T (Invitrogen, Carlsbad, CA, USA) containing a beta-lactamase reporter gene under the control of an UAS response element was used to screen Tox21 10K compound library. The cytotoxicity of the Tox21 compound library against the PR-bla cell line was tested in parallel by measuring the cell viability using CellTiter-Glo assay in the same wells.
Time in Hours:
24.00
Assay Format Type Sub:
cell-based format
Cell Free Component Source:
NA
Cell Growth Mode:
adherent