Assay Name:
TOX21_TRB_BLA_Antagonist_Followup
Organism:
human
Footprint:
microplate: 1536-well plate
Format:
cell-based
Cell Format:
cell line
Description:
The GeneBLAzer TRb-UAS-bla HEK293T assay kit for human TRb (ThermoFisherScientific Catalog No. K1684) was used as a secondary assay for specificity (TRb-bla).This assay contained the ligand-binding domain (LBD) of the human TRb fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer UAS-bla HEK293Tcell line. The reporter gene, b-lactamase, is under the transcriptional control of a UAS that binds the GAL4 DNA-binding domain. A cell viability assay was run in parallel for these cells as well using the CellTiter-Glo viability assay kit.
Time in Hours:
1.00
Assay Format Type Sub:
cell-based format
Cell Free Component Source:
NA
Cell Growth Mode:
adherent