Viewing Assay


Assay Name: CCTE_GLTED_hDIO1
Organism: human
Footprint: microplate: 96-well plate
Format: biochemical
Cell Format: cell-free
Description: CCTE_GLTED_hDIO1 is a cell-free, single-readout assay designed to test the inhibitory activity of chemicals toward human iodothyronine Deiodinase type 1 (DIO1) enzyme. Enzyme is incubated in presence of test chemical for 3 hours during which uninhibited enzyme liberates free iodide from substrate. Free iodide is measured in a second step using the Sandell-Kolthoff method.
Time in Hours: 3.00
Assay Format Type Sub: protein single format
Cell Free Component Source: HEK293
Cell Growth Mode: NA