Assay Name:
CCTE_GLTED_hDIO1
Organism:
human
Footprint:
microplate: 96-well plate
Format:
biochemical
Cell Format:
cell-free
Description:
CCTE_GLTED_hDIO1 is a cell-free, single-readout assay designed to test the inhibitory activity of chemicals toward human iodothyronine Deiodinase type 1 (DIO1) enzyme. Enzyme is incubated in presence of test chemical for 3 hours during which uninhibited enzyme liberates free iodide from substrate. Free iodide is measured in a second step using the Sandell-Kolthoff method.
Time in Hours:
3.00
Assay Format Type Sub:
protein single format
Cell Free Component Source:
HEK293
Cell Growth Mode:
NA