Viewing Assay


Assay Name: TOX21_PXR
Organism: human
Footprint: microplate: 1536-well plate
Format: cell-based
Cell Format: cell line
Description: The TOX21_PXR is a cell-based, single-readout assay that uses HepG2, a human liver cell line, with measurements taken at 24 hours after chemical dosing on a microplate: 1536-well plate. To screen Tox21 libraries for compounds that activate hPXR-mediated CYP3A4 gene expression, PXR-Luc HepG2 cells were used. Cytotoxicity of the compounds was tested in parallel by measuring the cell viability using CellTiter-Fluor assay system in the same wells.
Time in Hours: 24.00
Assay Format Type Sub: cell-based format
Cell Free Component Source: NA
Cell Growth Mode: adherent