Footprint:
microplate: 1536-well plate
Description:
The TOX21_PXR is a cell-based, single-readout assay that uses HepG2, a human liver cell line, with measurements taken at 24 hours after chemical dosing on a microplate: 1536-well plate. To screen Tox21 libraries for compounds that activate hPXR-mediated CYP3A4 gene expression, PXR-Luc HepG2 cells were used. Cytotoxicity of the compounds was tested in parallel by measuring the cell viability using CellTiter-Fluor assay system in the same wells.
Assay Format Type Sub:
cell-based format
Cell Free Component Source:
NA
Cell Growth Mode:
adherent