Assay Name:
TOX21_TRHR_HEK293
Organism:
human
Footprint:
microplate: 1536-well plate
Format:
cell-based
Cell Format:
cell line
Description:
TOX21_TRHR_HEK293 assay is a cell-based assay, single-readout assay that uses HEK293T, a human kidney cell line, with measurements taken at 24 hours after chemical dosing in a 1536-well plate. It has the potential to screen G protein-coupled receptors. A compound/drug can either activate (agonist) or suppress (antagonist) GPCRs that can be monitored by levels of intracellular calcium. Using this assay, the Tox21 10K compound library was screened to identify potential TRHR agonists.
Time in Hours:
20.00
Assay Format Type Sub:
cell-based format
Cell Free Component Source:
NA
Cell Growth Mode:
adherent