Assay Name:
TOX21_CASP3_CHO
Organism:
chinese hamster
Footprint:
microplate: 1536-well plate
Format:
cell-based
Cell Format:
cell line
Description:
The TOX21_CASP3_CHO is a cell-based, single-readout assay that uses CHO-K1, a hamster ovary cell line, with measurements taken at 16 hours after chemical dosing on a microplate: 1536-well plate. To identify small molecule compounds that induce apoptosis, a homogeneous, luminescent Caspase-Glo 3/7 assay was used. Wild type CHO-K1 cells were used to screen Tox21 libraries for identifying caspase-3/7 activity inducers. Cytotoxicity of the compounds was tested in parallel by measuring the cell viability using CellTiter-Fluor assay system in the same wells.
Time in Hours:
16.00
Assay Format Type Sub:
cell-based format
Cell Free Component Source:
NA
Cell Growth Mode:
adherent