Assay Name:
CCTE_Simmons_MITO
Footprint:
microplate: 96-well plate
Description:
CCTE_Simmons_MITO is cell-based, respirometric assay using HepG2 cells and a Seahorse XF Analyzer to measure oxygen consumption rates over four temporal phases spanning a total of 72 minutes: pre-injection (pre-treatment), basal (following test compound injection), maximal (following FCCP uncoupler injection), and inhibited (following rotenone/antimycin A injection)
Assay Format Type Sub:
cell-based format
Cell Free Component Source:
NA
Cell Growth Mode:
adherent