Assay Name:
UKN5_HCS_SBAD2
Organism:
human
Footprint:
microplate: 96-well plate
Format:
cell-based
Cell Format:
cell line
Description:
UKN5_HCS_SBAD2 is a cell-based, multiplexed-readout assay screening for neurite outgrowth and cell viability that uses SBAD2 (peripheral neurons differentiated from iPSC), a human peripheral nervous system cell line, with measurements taken at 3 hours after chemical dosing in a microplate: 96-well plate. UKN5, also referred to as PeriTox, is an assay that uses human iPSC line SBAD2 as a model of peripheral neurons. Following 24 hr chemical exposures in multi-well plates, neurite area is evaluated as a marker of neurite outgrowth using high-content imaging of cells stained with calcein-AM. Cell viability is assessed using stain Hoechst H-33342.
Time in Hours:
3.00
Assay Format Type Sub:
cell-based format
Cell Free Component Source:
NA
Cell Growth Mode:
adherent