Assay Name:
UKN2_HCS_IMR90
Organism:
human
Footprint:
microplate: 96-well plate
Format:
cell-based
Cell Format:
cell line
Description:
UKN2_HCS_IMR90 is a cell-based, multiplexed-readout assay screening for neural crest cell migration and cell viability that uses IMR90 (neural crest cells differentiated from iPSC), a human embryo cell line, with measurements taken at 3 hours after chemical dosing in a microplate: 96-well plate. UKN2, also known as cMINC, is an assay that uses IMR90 cells (neural crest cells differentiated from iPSC) as a model of neural crest cell migration. 24 hr after cell seeding, cell migration was initiated and proceeded for 24 hr. Then, chemical was applied, and after 24 hr of exposure migration and viability were assessed using high-content imaging of cells stained with calcein-AM and Hoechst H-33342.
Time in Hours:
3.00
Assay Format Type Sub:
cell-based format
Cell Free Component Source:
NA
Cell Growth Mode:
adherent