Assay Name:
UKN4_HCS_LUHMES
Organism:
human
Footprint:
microplate: 96-well plate
Format:
cell-based
Cell Format:
cell line
Description:
UKN4_HCS_LUHMES is a cell-based, multiplexed-readout assay screening for neurite outgrowth and cell viability that uses LUHMES, a human central nervous system cell line, with measurements taken at 3 hours after chemical dosing in a microplate: 96-well plate. UKN4, also referred to as NeuriTox, is an assay that uses LUHMES neuronal precursors as a model of dopaminergic neuronal differentiation. Following a 2-day differentiation period, cells are exposed to chemicals for 24 hr and neurite area is evaluated as a marker of neurite outgrowth using high-content imaging of cells stained with calcein AM. Cell viability is assessed using stain Hoechst H-33342.
Time in Hours:
3.00
Assay Format Type Sub:
cell-based format
Cell Free Component Source:
NA
Cell Growth Mode:
adherent