Assay Name:
IUF_NPC1
Organism:
human
Footprint:
microplate: 96-well plate
Format:
cell-based
Cell Format:
primary cell
Description:
IUF_NPC1 is a cell-based, multiplexed assay that uses hNPC, a human primary neural progenitor cells, with measurements taken at 72 hours after chemical dosing in a 96-well plate. Human neural progenitor cells (hNPC) are generated from human fetal brain cortex at gestational week (GW) 16-19. Grown in suspension culture and under proliferative conditions (proliferation media and growth factors), these cells represent neural progenitor cell proliferation. In the NPC1 assay, hNPCs are exposed to the test compound for 72h. Cell proliferation is assessed as an increase in sphere area using automated phase contrast imaging and as incorporation of Bromodeoxyuridine (BrdU) during DNA synthesis using a luminescence-based cell proliferation ELISA. In parallel, the cell viability is assessed using an alamar blue viability assay and the cytotoxicity using a lactate dehydrogenase dependent membrane integrity assay.
Time in Hours:
72.00
Assay Format Type Sub:
cell-based format
Cell Free Component Source:
neural progenitor cells
Cell Growth Mode:
suspension