Assay Name:
IUF_NPC2-5
Organism:
human
Footprint:
microplate: 96-well plate
Format:
cell-based
Cell Format:
primary cell
Description:
IUF_NPC2-5 is a cell-based, multiplexed assay that uses hNPC, a human primary neural progenitor cells, with measurements taken at 72 or 120 hours after chemical dosing in a 96-well plate. Human neural progenitor cells (hNPC) are generated from human fetal brain cortex at gestational week (GW) 16-19. In the neural progenitor cell migration and differentiation assay (NPC2-5), hNPC’s grown as spheres are plated on an extracellular matrix and migrate and differentiate out of the sphere core. The processes radial glia migration, neuronal and oligodendrocyte migration as well as neuronal differentiation, neuronal morphology and oligodendrocyte differentiation are studied using automated phase contrast imaging and automated fluorescence imaging in combination with high content image analysis. In parallel, the cell viability is assessed using an alamar blue viability assay and the cytotoxicity using a lactate dehydrogenase dependent membrane integrity assay.
Time in Hours:
72.00
Assay Format Type Sub:
cell-based format
Cell Free Component Source:
neural progenitor cells
Cell Growth Mode:
suspension